1887

Abstract

Two bacterial strains, DNG5 and V3M1, isolated from forest soil of the Changbai mountains in China, were characterized using a polyphasic approach. Analysis of their 16S rRNA gene sequences indicated that strains DNG5 and V3M1 were phylogenetically related to members of the genus (96.0–98.4 % similarity) and (96.7–98.0 % similarity), respectively, within the order . Strains DNG5 and V3M1 were Gram-stain-positive and strictly aerobic and formed yellow colonies on LB agar. Cells of strain DNG5 were short, non-motile rods, 0.4–0.5×0.8–1.0 μm. Strain DNG5 contained MK-10 and MK-11 as the major respiratory quinones and anteiso-C (49.2 %) and iso-C (22.4 %) as the major fatty acids. The diamino acid in the peptidoglycan of strain DNG5 was 2,4-diaminobutyric acid and the murein was of the acetyl type. Cells of strain V3M1 were cocci, 0.6–0.7 μm in diameter. The cell-wall peptidoglycan of strain V3M1 contained the amino acids lysine, glutamic acid, alanine and glycine. Strain V3M1 contained MK-7, MK-7(H), MK-8 and MK-8(H) as respiratory quinones and anteiso-C (78.2 %) and iso-C (13.1 %) as the major cellular fatty acids. The DNA G+C contents of strains DNG5 and V3M1 were 75.9 and 67.2 mol%, respectively. The DNA–DNA relatedness of strain DNG5 to DSM 22002, JCM 9950, JCM 12132 and JCM 12398 was 58.3, 43.9, 36.1 and 54.1 %, respectively. The DNA–DNA relatedness of strain V3M1 to CGMCC 1.2299, CGMCC 1.2373 and CGMCC 1.2300 was 57.5, 45.4 and 39.0 %, respectively. Combining phenotypic and genotypic traits, strain DNG5 represents a novel species of the genus , for which the name sp. nov. is proposed, with DNG5 (=CGMCC 1.6960 =NBRC 104260) as the type strain. Strain V3M1 represents a novel species of the genus , for which the name sp. nov. is proposed, with V3M1 (=CGMCC 1.7054 =NBRC 104258) as the type strain.

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2010-08-01
2020-01-18
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Polar lipids of strain DNG5 after separation by two-dimensional TLC. DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; GL1 and GL2, unknown glycolipids. (a) Phospholipids; (b) glycolipids.

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Polar lipids of strain V3M1 after separation by two-dimensional TLC. DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; PI, phosphatidylinositol; PL, unindentified phospholipid that showed similar migration behaviour to phosphatidylcholine; GL1, GL2, and GL3, unknown glycolipids. (a) Phospholipids; (b) glycolipids.

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