1887

Abstract

A novel carbendazim-degrading actinobacterium, designated djl-6-2, was isolated from the sludge of a carbendazim wastewater treatment facility in Jiangsu province, China. The morphological and chemotaxonomic properties of the isolate were typical of members of the genus . Strain djl-6-2 formed a coherent cluster with djl-6, DSM 44587, DSM 43066 and DSM 43954 in 16S rRNA gene sequence analysis. The results of DNA–DNA hybridization with the above strains (27.7, 19.3, 18.6 and 10.6 % relatedness, respectively), in combination with differences in biochemical and physiological properties, suggest that strain djl-6-2 should be classified within a novel species of the genus , for which the name sp. nov. is proposed, with djl-6-2 (=DSM 45257 =CCTCC AB 208292) as the type strain.

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2010-02-01
2024-11-03
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References

  1. Bell K. S., Philp J., Aw D., Christofi N. 1998; The genus Rhodococcus . J Appl Microbiol 85:195–210 [CrossRef]
    [Google Scholar]
  2. Buck J. D. 1982; Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993
    [Google Scholar]
  3. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  4. Collins M. D., Jones D. 1980; Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48:459–470 [CrossRef]
    [Google Scholar]
  5. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  7. Ebersole L. L. 1992; Acid-fast stain procedures. In Clinical Microbiology Procedures Handbook pp. 3.5.1–3.5.11 Edited by Isenberg H. D. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  8. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  9. Ghosh A., Paul D., Prakash D., Mayilraj S., Jain R. K. 2006; Rhodococcus imtechensis sp. nov., a nitrophenol-degrading actinomycete. Int J Syst Evol Microbiol 56:1965–1969 [CrossRef]
    [Google Scholar]
  10. Goodfellow M. 1986; Genus Rhodococcus Zopf 1891, 28AL . In Bergey's Manual of Systematic Bacteriology vol 2 pp 1472–1481 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  11. Goodfellow M., Alderson G., Chun J. 1998; Rhodococcal systematics: problems and developments. Antonie van Leeuwenhoek 74:3–20 [CrossRef]
    [Google Scholar]
  12. Goodfellow M., Isik K., Yates E. 1999; Actinomycete systematics: an unfinished synthesis. Nova Acta Leopold 312:47–82
    [Google Scholar]
  13. Gordon R. E. 1966; Some criteria for the recognition of Nocardia madurae (Vincent) Blanchard. J Gen Microbiol 45:355–364 [CrossRef]
    [Google Scholar]
  14. Gordon R. E. 1967; The taxonomy of soil bacteria. In The Ecology of Soil Bacteria pp 293–321 Edited by Gray T. R. G., Parkinson B. Liverpool: Liverpool University Press;
    [Google Scholar]
  15. Holtman M. A., Kobayashi D. Y. 1997; Identification of Rhodococcus erythropolis isolates capable of degrading the fungicide carbendazim. Appl Microbiol Biotechnol 47:578–582 [CrossRef]
    [Google Scholar]
  16. Kimura M. 1983 The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press;
    [Google Scholar]
  17. Kroppenstedt R. M. 1982; Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5:2359–2367 [CrossRef]
    [Google Scholar]
  18. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; mega2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  19. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  20. Li Y., Kawamura Y., Fujiwara N., Naka T., Liu H. S., Huang X. X., Kobayashi K., Ezaki T. 2004 Rothia aeria sp. nov., Rhodococcus baikonurensis sp. nov. and Arthrobacter russicus sp. nov., isolated from air in the Russian space laboratory Mir. Int J Syst Evol Microbiol 54, 827–835. [CrossRef]
  21. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  22. Mazellier P., Leroy É., De Laat J., Legube B. 2003; Degradation of carbendazim by UV/H2O2 investigated by kinetic modelling. Environ Chem Lett 1:68–72 [CrossRef]
    [Google Scholar]
  23. Minnikin D. E., Collins M. D., Goodfellow M. 1979; Fatty acid and polar lipid composition in the classification of Cellulomonas , Oerskovia and related taxa. J Appl Bacteriol 47:87–95 [CrossRef]
    [Google Scholar]
  24. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr A 188:221–233 [CrossRef]
    [Google Scholar]
  25. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  26. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  27. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:16
    [Google Scholar]
  28. Shirling E. D., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  29. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology . pp 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  30. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  31. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  32. Warhurst A. M., Fewson C. A. 1994; Biotransformations catalyzed by the genus Rhodococcus . Crit Rev Biotechnol 14:29–73 [CrossRef]
    [Google Scholar]
  33. Xu J. L., Gu X. Y., Shen B., Wang Z. C., Wang K., Li S. P. 2006a Isolation and characterization of a carbendazim-degrading Rhodococcus sp. djl-6. Curr Microbiol 53, 72–76. [CrossRef]
  34. Xu J. L., Wang Z. C., Wang K., Li S. P. 2006b; The isolation, identification and degradation characters of an efficient carbendazim-degrading bacterium. China Environ Sci 26:307–310 (in Chinese
    [Google Scholar]
  35. Xu J. L., He J., Wang Z. C., Wang K., Li W. J., Tang S. K., Li S. P. 2007; Rhodococcus qingshengii sp. nov., a carbendazim-degrading bacterium. Int J Syst Evol Microbiol 57:2754–2757 [CrossRef]
    [Google Scholar]
  36. Yassin A. F. 2005; Rhodococcus triatomae sp. nov., isolated from a blood-sucking bug. Int J Syst Evol Microbiol 55:1575–1579 [CrossRef]
    [Google Scholar]
  37. Yoon J. H., Cho Y. G., Kang S. S., Kim S. B., Lee S. T., Park Y. H. 2000; Rhodococcus koreensis sp. nov., a 2,4-dinitrophenol-degrading bacterium. Int J Syst Evol Microbiol 50:1193–1201 [CrossRef]
    [Google Scholar]
  38. Zhang J., Zhang Y., Xiao C., Liu Z., Goodfellow M. 2002; Rhodococcus maanshanensis sp. nov., a novel actinomycete from soil. Int J Syst Evol Microbiol 52:2121–2126 [CrossRef]
    [Google Scholar]
  39. Zhang G. S., Jia X. M., Cheng T. F., Ma X. H., Zhao Y. H. 2005a; Isolation and characterization of a new carbendazim-degrading Ralstonia sp. strain. World J Microbiol Biotechnol 21:265–269 [CrossRef]
    [Google Scholar]
  40. Zhang Y. Q., Li W. J., Kroppenstedt R. M., Kim C. J., Chen G. Z., Park D. J., Xu L. H., Jiang C. L. 2005b; Rhodococcus yunnanensis sp. nov., a mesophilic actinobacterium isolated from forest soil. Int J Syst Evol Microbiol 55:1133–1137 [CrossRef]
    [Google Scholar]
  41. Zopf W. 1891; Über Ausscheidung von Fettfarbstoffen (Lipochromen) seitens gewisser Spaltpilze. Ber Dtsch Bot Ges 9:22–28 (in German)
    [Google Scholar]
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