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Abstract

A polycyclic aromatic hydrocarbon-degrading marine bacterium, designated strain P-4, was isolated from oil-polluted saline soil in Xianhe, Shangdong Province, China. Strain P-4 was Gram-negative-staining with curved to spiral rod-shaped cells and grew optimally with 3–6 % (w/v) NaCl and at 30 °C. The predominant fatty acids were C 7 (35.0 %), C (25.0 %), C 7 (17.9 %), C (6.2 %) and C cyclo (5.2 %). The major respiratory quinone was Q-9 and the genomic DNA G+C content was 61.2±1.0 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain P-4 belonged to the genus of the class . DNA–DNA hybridization with DSM 17429 showed relatedness of 36.0 %, and lower values were obtained with respect to other species. Based on physiological and biochemical tests and 16S rRNA gene sequence analysis as well as DNA–DNA relatedness, strain P-4 should be placed in the genus within a novel species. The name sp. nov. is proposed, with P-4 (=CGMCC 1.6849 =JCM 14850) as the type strain.

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2010-05-01
2019-09-16
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vol. , part 5, pp. 1125 - 1129

Cellular fatty acid contents of strain P-4 and related type strains of the genus .

DNA–DNA relatedness between strain P-4 and type strains of species.

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