A Gram-positive, non-motile and coccoid-, short rod- or rod-shaped bacterial strain, ISL-16T, was isolated from a marine solar saltern in Korea and its taxonomic position was investigated using a polyphasic taxonomic approach. Strain ISL-16T grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISL-16T joined the cluster comprising species of the genus Planococcus. Its 16S rRNA gene sequence contained the same signature nucleotides as those defined for the genus Planococcus. Strain ISL-16T exhibited 16S rRNA gene sequence similarity values of 96.9–98.2 % to the type strains of species of the genus Planococcus. Strain ISL-16T contained MK-8 and MK-7 as the predominant menaquinones and anteiso-C15 : 0, C16 : 1ω7c alcohol and anteiso-C17 : 0 as the major fatty acids. The DNA G+C content was 48.3 mol%. DNA–DNA relatedness values between strain ISL-16T and the type strains of species of the genus Planococcus were 15–28 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, enabled strain ISL-16T to be differentiated from recognized species of the genus Planococcus. On the basis of the data presented, strain ISL-16T is considered to represent a novel species of the genus Planococcus, for which the name Planococcus salinarum sp. nov. is proposed. The type strain is ISL-16T (=KCTC 13584T=CCUG 57753T). An emended description of the genus Planococcus is also given.
BrunsA.,
RohdeM.,
Berthe-CortiL.2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006[CrossRef]
ChoiJ.-H.,
ImW.-T.,
LiuQ.-M.,
YooJ.-S.,
ShinJ.-H.,
RheeS.-K.,
RohD.-H.2007; Planococcus donghaensis sp. nov., a starch-degrading bacterium isolated from the East Sea, South Korea. Int J Syst Evol Microbiol 57:2645–2650[CrossRef]
DaiX.,
WangY.-N.,
WangB.-J.,
LiuS.-J.,
ZhouY.-G.2005; Planomicrobium chinense sp. nov., isolated from coastal sediment, and transfer of Planococcus psychrophilus and Planococcus alkanoclasticus to Planomicrobium as Planomicrobium psychrophilum comb. nov. and Planomicrobium alkanoclasticum comb. nov. Int J Syst Evol Microbiol 55:699–702[CrossRef]
EzakiT.,
HashimotoY.,
YabuuchiE.1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229[CrossRef]
HaoM. V.,
KomagataK.1985; A new species of Planococcus , P. kocurii isolated from fish, frozen foods, and fish curing brine. J Gen Appl Microbiol 31:441–455[CrossRef]
JungY.-T.,
KangS.-J.,
OhT.-K.,
YoonJ.-H.,
KimB.-H.2009; Planomicrobium flavidum sp. nov., isolated from a marine solar saltern, and transfer of Planococcus stackebrandtii Mayilraj et al. 2005 to the genus Planomicrobium as Planomicrobium stackebrandtii comb. nov. Int J Syst Evol Microbiol 59:2929–2933[CrossRef]
MayilrajS.,
PrasadG. S.,
SureshK.,
SainiH. S.,
ShivajiS.,
ChakrabartiT.2005; Planococcus stackebrandtii sp. nov., isolated from a cold desert of the Himalayas, India. Int J Syst Evol Microbiol 55:91–94[CrossRef]
NakagawaY.,
SakaneT.,
YokotaA.1996; Emendation of the genus Planococcus and transfer of Flavobacterium okeanokoites Zobell and Upham 1944 to the genus Planococcus as Planococcus okeanokoites comb. nov. Int J Syst Bacteriol 46:866–870[CrossRef]
ReddyG. S. N.,
PrakashJ. S. S.,
VairamaniM.,
PrabhakarS.,
MatsumotoG. I.,
ShivajiS.2002; Planococcus antarcticus and Planococcus psychrophilus spp. nov. isolated from cyanobacterial mat samples collected from ponds in Antarctica. Extremophiles 6:253–261[CrossRef]
RomanoI.,
GiordanoA.,
LamaL.,
NicolausB.,
GambacortaA.2003; Planococcus rifietensis sp. nov., isolated from algal mat collected from a sulfurous spring in Campania (Italy. Syst Appl Microbiol 26:357–366[CrossRef]
StackebrandtE.,
GoebelB. M.1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849[CrossRef]
TamaokaJ.,
KomagataK.1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128[CrossRef]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E.other authors1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464[CrossRef]
YoonJ.-H.,
KimH.,
KimS.-B.,
KimH.-J.,
KimW. Y.,
LeeS. T.,
GoodfellowM.,
ParkY.-H.1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505[CrossRef]
YoonJ.-H.,
LeeS. T.,
ParkY.-H.1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194[CrossRef]
YoonJ.-H.,
KangS.-S.,
LeeK.-C.,
KhoY. H.,
KangK. H.,
ParkY.-H.2001; Planomicrobium koreense gen. nov., sp. nov., a bacterium isolated from the Korean traditional fermented seafood jeotgal, and transfer of Planococcus okeanokoites (Nakagawa et al . 1996) and Planococcus mcmeekinii (Junge et al . 1998) to the genus Planomicrobium
. Int J Syst Evol Microbiol 51:1511–1520
YoonJ.-H.,
WeissN.,
KangK. H.,
OhT.-K.,
ParkY.-H.2003a; Planococcus maritimus sp. nov., isolated from sea water of a tidal flat in Korea. Int J Syst Evol Microbiol 53:2013–2017[CrossRef]
YoonJ.-H.,
KangK. H.,
ParkY.-H.2003b; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454[CrossRef]