1887

Abstract

A Gram-positive, non-motile and coccoid-, short rod- or rod-shaped bacterial strain, ISL-16, was isolated from a marine solar saltern in Korea and its taxonomic position was investigated using a polyphasic taxonomic approach. Strain ISL-16 grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISL-16 joined the cluster comprising species of the genus . Its 16S rRNA gene sequence contained the same signature nucleotides as those defined for the genus . Strain ISL-16 exhibited 16S rRNA gene sequence similarity values of 96.9–98.2 % to the type strains of species of the genus . Strain ISL-16 contained MK-8 and MK-7 as the predominant menaquinones and anteiso-C, C 7 alcohol and anteiso-C as the major fatty acids. The DNA G+C content was 48.3 mol%. DNA–DNA relatedness values between strain ISL-16 and the type strains of species of the genus were 15–28 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, enabled strain ISL-16 to be differentiated from recognized species of the genus . On the basis of the data presented, strain ISL-16 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is ISL-16 (=KCTC 13584=CCUG 57753). An emended description of the genus is also given.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.013136-0
2010-04-01
2021-04-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/4/754.html?itemId=/content/journal/ijsem/10.1099/ijs.0.013136-0&mimeType=html&fmt=ahah

References

  1. Alam S. I., Singh L., Dube S., Reddy G. S. N., Shivaji S. 2003; Psychrophilic Planococcus maitriensis sp. nov. from Antarctica. Syst Appl Microbiol 26:505–510 [CrossRef]
    [Google Scholar]
  2. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [CrossRef]
    [Google Scholar]
  3. Choi J.-H., Im W.-T., Liu Q.-M., Yoo J.-S., Shin J.-H., Rhee S.-K., Roh D.-H. 2007; Planococcus donghaensis sp. nov., a starch-degrading bacterium isolated from the East Sea, South Korea. Int J Syst Evol Microbiol 57:2645–2650 [CrossRef]
    [Google Scholar]
  4. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  5. Dai X., Wang Y.-N., Wang B.-J., Liu S.-J., Zhou Y.-G. 2005; Planomicrobium chinense sp. nov., isolated from coastal sediment, and transfer of Planococcus psychrophilus and Planococcus alkanoclasticus to Planomicrobium as Planomicrobium psychrophilum comb. nov. and Planomicrobium alkanoclasticum comb. nov. Int J Syst Evol Microbiol 55:699–702 [CrossRef]
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  7. Hao M. V., Komagata K. 1985; A new species of Planococcus , P. kocurii isolated from fish, frozen foods, and fish curing brine. J Gen Appl Microbiol 31:441–455 [CrossRef]
    [Google Scholar]
  8. Jung Y.-T., Kang S.-J., Oh T.-K., Yoon J.-H., Kim B.-H. 2009; Planomicrobium flavidum sp. nov., isolated from a marine solar saltern, and transfer of Planococcus stackebrandtii Mayilraj et al. 2005 to the genus Planomicrobium as Planomicrobium stackebrandtii comb. nov. Int J Syst Evol Microbiol 59:2929–2933 [CrossRef]
    [Google Scholar]
  9. Kocur M., Pácová Z., Hodgkiss W., Martinec T. 1970; The taxonomic status of the genus Planococcus Migula 1894. Int J Syst Bacteriol 20:241–248 [CrossRef]
    [Google Scholar]
  10. Komagata K., Suzuki K. 1987; Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  11. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  12. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  13. Mayilraj S., Prasad G. S., Suresh K., Saini H. S., Shivaji S., Chakrabarti T. 2005; Planococcus stackebrandtii sp. nov., isolated from a cold desert of the Himalayas, India. Int J Syst Evol Microbiol 55:91–94 [CrossRef]
    [Google Scholar]
  14. Migula W. 1894; Über ein neues System der Bakterien. Arb Bakteriol Inst Karlsruhe 1:235–238 (in German
    [Google Scholar]
  15. Nakagawa Y., Sakane T., Yokota A. 1996; Emendation of the genus Planococcus and transfer of Flavobacterium okeanokoites Zobell and Upham 1944 to the genus Planococcus as Planococcus okeanokoites comb. nov. Int J Syst Bacteriol 46:866–870 [CrossRef]
    [Google Scholar]
  16. Reddy G. S. N., Prakash J. S. S., Vairamani M., Prabhakar S., Matsumoto G. I., Shivaji S. 2002; Planococcus antarcticus and Planococcus psychrophilus spp. nov. isolated from cyanobacterial mat samples collected from ponds in Antarctica. Extremophiles 6:253–261 [CrossRef]
    [Google Scholar]
  17. Romano I., Giordano A., Lama L., Nicolaus B., Gambacorta A. 2003; Planococcus rifietensis sp. nov., isolated from algal mat collected from a sulfurous spring in Campania (Italy. Syst Appl Microbiol 26:357–366 [CrossRef]
    [Google Scholar]
  18. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  19. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  20. Suresh K., Mayilraj S., Bhattacharya A., Chakrabarti T. 2007; Planococcus columbae sp. nov., isolated from pigeon faeces. Int J Syst Evol Microbiol 57:1266–1271 [CrossRef]
    [Google Scholar]
  21. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  22. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  23. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  24. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
  25. Yoon J.-H., Kang S.-S., Lee K.-C., Kho Y. H., Kang K. H., Park Y.-H. 2001; Planomicrobium koreense gen. nov., sp. nov., a bacterium isolated from the Korean traditional fermented seafood jeotgal, and transfer of Planococcus okeanokoites (Nakagawa et al . 1996) and Planococcus mcmeekinii (Junge et al . 1998) to the genus Planomicrobium . Int J Syst Evol Microbiol 51:1511–1520
    [Google Scholar]
  26. Yoon J.-H., Weiss N., Kang K. H., Oh T.-K., Park Y.-H. 2003a; Planococcus maritimus sp. nov., isolated from sea water of a tidal flat in Korea. Int J Syst Evol Microbiol 53:2013–2017 [CrossRef]
    [Google Scholar]
  27. Yoon J.-H., Kang K. H., Park Y.-H. 2003b; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454 [CrossRef]
    [Google Scholar]
  28. Zhang D.-C., Liu H.-C., Xin Y.-H., Yu Y., Zhou P.-J., Zhou Y. G. 2009; Planomicrobium glaciei sp. nov., a psychrotolerant bacterium isolated from a glacier. Int J Syst Evol Microbiol 59:1387–1390 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.013136-0
Loading
/content/journal/ijsem/10.1099/ijs.0.013136-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error