1887

Abstract

Three strains of a hitherto unknown, Gram-negative, tiny, anaerobic coccus were collected from human clinical samples originating from skin and soft tissues. The three isolates displayed at least 99.9 % identity in their 16S rRNA gene sequences and more than 99.8 % identity in their gene sequences. The isolates were affiliated to the family , the coccobacillus being the most closely related species, but there was no more than 91.1 % identity in the 16S rRNA gene sequence between this species and the three isolates. Phylogeny based on the 16S rRNA gene confirmed that the three strains represent a novel and robust lineage within the current family . A similar genomic structure was demonstrated for the three isolates by PFGE-based analysis. Morphology and metabolic end products, as well as genotypic and phylogenetic data supported the proposal of the novel genus gen. nov., with the novel species sp. nov. [type strain ADV 07/08/06-B-1388 (=AIP 149.07=CIP 109806=DSM 21255=CCUG 56017) as type species]. Phylogenetic analyses based on the 16S rRNA gene sequences of members of the phylum and other phyla indicated that the family forms a robust lineage clearly separated from those of the classes ‘’, ‘’, and ‘’ in the phylum . Therefore, we propose that this family is a class-level taxon in the phylum , for which the name classis nov. is proposed, based on the Gram-negative type of cell wall of its members, with the type order ord. nov. In this order, a novel family, fam. nov., is proposed and description of the family is emended.

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2010-06-01
2021-10-20
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