1887

Abstract

A Gram-negative, non-motile, pale-yellow, rod-shaped bacterial strain, DS-42, was isolated from a soil in Korea and its taxonomic position was investigated by a polyphasic study. Strain DS-42 grew optimally at 25 °C and pH 7.0–8.0. Strain DS-42 did not form nodules on three different legumes, and the and genes were also not detected by PCR. Strain DS-42 contained Q-10 as the predominant ubiquinone. The major cellular fatty acid was C 7. The DNA G+C content was 60.8 mol%. Phylogenetic analyses based on 16S rRNA, and gene sequences showed that strain DS-42 belonged to the genus . Strain DS-42 showed 16S rRNA gene sequence similarity of 94.1–97.7 % to the type strains of recognized species. DNA–DNA relatedness between strain DS-42 and the type strains of , , and was 13–19 %, indicating that strain DS-42 was distinct from them genetically. Strain DS-42 can also be differentiated from these four phylogenetically related species by various phenotypic properties. On the basis of phenotypic properties, phylogenetic distinctiveness and genetic data, strain DS-42 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DS-42 (=KCTC 12873 =JCM 14591).

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2010-06-01
2019-10-23
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vol. , part 6, pp. 1387 - 1393

Neighbour-joining trees showing the phylogenetic positions of sp. nov. DS-42 and other related taxa based on partial and gene sequences. [PDF](34 KB)



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