Strain 56T was isolated from a hypersaline soil in Aswan (Egypt). Cells were pleomorphic rods. The organism was neutrophilic, motile and required at least 1.7 M (10 % w/v) NaCl, but not MgCl2, for growth; optimal growth occurred at ≥3.8 M (≥22.5 %) NaCl. The strain was thermotolerant with an optimum temperature for growth of 40 °C, although growth was possible up to 55 °C. The G+C content of the DNA of the novel strain was 67.1 mol%.16S rRNA gene sequence analysis revealed that strain 56T was a member of the phyletic group defined by the family Halobacteriaceae, showing the highest similarity to Halopiger xanaduensis SH-6T (99 %) and the next highest similarity of 94 % to other members of the family Halobacteriaceae. DNA–DNA hybridization revealed 27 % relatedness between strain 56T and Hpg. xanaduensis SH-6T. Polar lipid analysis revealed the presence of the bis-sulfated glycolipid S2-DGD-1 as the sole glycolipid and the absence of the glycerol diether analogue phosphatidylglycerosulfate. Both C20 . 20 and C20 . 25 core lipids were present. Strain 56T accumulated large amounts of polyhydroxybutyrate and also secreted an exopolymer. Physiological and biochemical differences suggested that Hpg. xanaduanesis and strain 56T were sufficiently different to be separated into two distinct species. It is suggested that strain 56T represents a novel species of the genus Halopiger, for which the name Halopiger aswanensis sp. nov. is proposed. The type strain is strain 56T (=DSM 13151T=JCM 11628T).
AntónJ.,
MeseguerI.,
Rodríguez-ValeraF.1988; Production of an extracellular polysaccharide by Haloferax mediterranei
. Appl Environ Microbiol 54:2381–2386
ArahalD. R.,
GarcíaM. T.,
LudwigW.,
SchleiferK. H.,
VentosaA.2001; Transfer of Halomonas canadensis and Halomonas israelensis to the genus Chromohalobacter as Chromohalobacter canadensis comb. nov. and Chromohalobacter israelensis comb. nov. Int J Syst Evol Microbiol 51:1443–1448
CashionP.,
Holder-FranklinM. A.,
McCullyJ.,
FranklinM.1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466[CrossRef]
Fernandez-CastilloR.,
Rodriguez-ValeraF.,
Gonzales-RamosJ.,
Ruiz-BerraqueroF.1986; Accumulation of poly( β -hydroxybutyrate) by halobacteria. Appl Environ Microbiol 51:214–216
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
(editors) 1994Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
GutiérrezM. C.,
CastilloA. M.,
KamekuraM.,
XueY.,
MaY.,
CowanD. A.,
JonesB. E.,
GrantW. D.,
VentosaA.2007Halopiger xanaduensis gen. nov., sp. nov., an extremely halophilic archaeon isolated from saline Lake Shangmatala in Inner Mongolia, China. Int J Syst Evol Microbiol 57, 1402–1407 [CrossRef]
HezayenF. F.,
RehmB. H. A.,
EberhardtR.,
SteinbüchelA.2000; Polymer production by two newly isolated extremely halophilic Archaea : Application of a novel corrosion-resistant bioreactor. Appl Microbiol Biotechnol 54:319–325[CrossRef]
HezayenF. F.,
RehmB. H. A.,
TindallB. J.,
SteinbüchelA.2001; Transfer of Natrialba asiatica B1T to Natrialba taiwanensis sp. nov. and description of Natrialba aegyptiaca sp. nov., a novel extremely halophilic, aerobic, non-pigmented member of the Archaea from Egypt which produces extracellular poly(glutamic acid). Int J Syst Evol Microbiol 51:1133–1142[CrossRef]
HezayenF. F.,
TindallB. J.,
SteinbüchelA.,
RehmB. H. A.2002a; Characterization of a novel halophilic archaeon Halobiforma haloterrestera gen. nov., sp. nov. and transfer of Natronobacterium nitratireducens to Halobiforma nitratireducens comb. nov. Int J Syst Evol Microbiol 52:2271–2280[CrossRef]
HezayenF. F.,
SteinbüchelA.,
RehmB. H. A.2002b; Biochemical and enzymological properties of the polyhydroxybutyrate synthase from the extremely halophilic archaeon strain 56. Arch Biochem Biophys 403:284–291[CrossRef]
JohnsonJ. L.1994; Similarity analysis of DNAs. In Methods for General and Molecular Bacteriology
. pp 655–682 Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
Washington, DC: American Society for Microbiology;
LilloJ. G.,
Rodriguez-ValeraF.1990; Effects of culture conditions on poly( β -hydroxybutyric acid) production by Haloferax mediterranei
. Appl Environ Microbiol 56:2517–2521
MakY. M.,
HoK. K.1992; An improved method for the isolation of chromosomal DNA from various bacteria and cyanobacteria. Nucleic Acids Res 20:4101–4102[CrossRef]
MesbahM.,
PremachandranU.,
WhitmanW. B.1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167[CrossRef]
NicolausB.,
LamaL.,
EspositoE.,
MancaM. C.,
ImprotaR.,
BellittiM. R.,
DuckworthA. W.,
GrantW. D.,
GambacortaA.1999; Haloarcula spp. able to biosynthesize exo- and endopolymers. J Ind Microbiol Biotechnol 23:489–496[CrossRef]
OrenA.,
VentosaA.,
GrantW. D.1997; Proposed minimal standards for description of new taxa in the order Halobacteriales
. Int J Syst Bacteriol 47:233–238[CrossRef]
ParamonovN. A.,
ParolisL. A. S.,
ParolisH.,
BóanI. F.,
AntónJ.,
Rodríguez-ValeraF.1998; The structure of the exocellular polysaccharide produced by the Archaeon Haloferax gibbonsii (ATCC 33959). Carbohydr Res 309:89–94[CrossRef]
ParolisL. A. S.,
ParolisH.,
ParamonovN. A.,
BóanI. F.,
AntónJ.,
Rodríguez-ValeraF.1999; Structural studies on the acidic exopolysaccharide from Haloferax denitrificans ATCC 35960. Carbohydr Res 319:133–140[CrossRef]
StackebrandtE.,
GoebelB. M.1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849
ThompsonJ. D.,
HigginsD. G.,
GibsonT. J.1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680[CrossRef]
TindallB. J.1990a; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130[CrossRef]