1887

Abstract

A novel, extremely halophilic archaeon, D43, was isolated from traditional salt-fermented seafood in Korea. The cells were Gram-negative-staining and motile. The strain grew at 15–50 °C, 10–30 % (w/v) NaCl and pH 6.0–8.0. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain D43 is affiliated with the family in the domain and had 95.5 % 16S rRNA gene sequence similarity with DX253. The sequence from strain D43 formed a clade with those from regardless of which tree-generating algorithm was used. DNA–DNA hybridization experiments showed 25.8 % relatedness between the isolate and KCTC 4006. Major lipids were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two unidentified glycolipids. The DNA G+C content of the isolate was 56.5 mol%. On the basis of this polyphasic taxonomic study, strain D43 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is D43 (=DSM 19505 =JCM 15962).

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2010-05-01
2020-01-26
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References

  1. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  2. Bauer, A. W., Kirby, W. M., Sherris, J. C. & Turck, M. ( 1966; ). Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45, 493–496.
    [Google Scholar]
  3. Dittmer, J. C. & Lester, R. L. ( 1964; ). A simple, specific spray for the detection of phospholipids on thin-layer chromatograms. J Lipid Res 15, 126–127.
    [Google Scholar]
  4. Dussault, H. P. ( 1955; ). An improved technique for staining red halophilic bacteria. J Bacteriol 70, 484–485.
    [Google Scholar]
  5. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 2005; ). phylip (phylogeny inference package), version 3.6. Distributed by the author. University of Washington, Seattle, USA.
  8. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  9. Gonzalez, J. M. & Saiz-Jimenez, C. ( 2002; ). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4, 770–773.[CrossRef]
    [Google Scholar]
  10. Gutierrez, C. & Gonzalez, C. ( 1972; ). Method for simultaneous detection of proteinase and esterase activities in extremely halophilic bacteria. Appl Microbiol 24, 516–517.
    [Google Scholar]
  11. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  12. Kluge, A. G. & Farris, J. S. ( 1969; ). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef]
    [Google Scholar]
  13. Oren, A., Duker, S. & Ritter, S. ( 1996; ). The polar lipid composition of Walsby's square bacterium. FEMS Microbiol Lett 138, 135–140.[CrossRef]
    [Google Scholar]
  14. Oren, A., Ventosa, A. & Grant, W. D. ( 1997; ). Proposed minimal standards for description of new taxa in the order Halobacteriales. Int J Syst Bacteriol 47, 233–238.[CrossRef]
    [Google Scholar]
  15. Purdy, K. J., Cresswell-Maynard, T. D., Nedwell, D. B., McGenity, T. J., Grant, W. D., Timmis, K. N. & Embley, T. M. ( 2004; ). Isolation of haloarchaea that grow at low salinities. Environ Microbiol 6, 591–595.[CrossRef]
    [Google Scholar]
  16. Roh, S. W. & Bae, J. W. ( 2009; ). Halorubrum cibi sp. nov., an extremely halophilic archaeon from salt-fermented seafood. J Microbiol 47, 162–166.[CrossRef]
    [Google Scholar]
  17. Roh, S. W., Nam, Y.-D., Chang, H.-W., Kim, K.-H., Lee, H.-J., Oh, H.-M. & Bae, J.-W. ( 2007a; ). Natronococcus jeotgali sp. nov., a halophilic archaeon isolated from shrimp jeotgal, a traditional fermented seafood from Korea. Int J Syst Evol Microbiol 57, 2129–2131.[CrossRef]
    [Google Scholar]
  18. Roh, S. W., Nam, Y. D., Chang, H. W., Sung, Y., Kim, K. H., Oh, H. M. & Bae, J. W. ( 2007b; ). Halalkalicoccus jeotgali sp. nov., a halophilic archaeon from shrimp jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 57, 2296–2298.[CrossRef]
    [Google Scholar]
  19. Roh, S. W., Sung, Y., Nam, Y. D., Chang, H. W., Kim, K. H., Yoon, J. H., Jeon, C. O., Oh, H. M. & Bae, J. W. ( 2008; ). Arthrobacter soli sp. nov., a novel bacterium isolated from wastewater reservoir sediment. J Microbiol 46, 40–44.[CrossRef]
    [Google Scholar]
  20. Roh, S. W., Nam, Y.-D., Chang, H.-W., Kim, K.-H., Sung, Y., Kim, M.-S., Oh, H.-M. & Bae, J.-W. ( 2009; ). Haloterrigena jeotgali sp. nov., an extremely halophilic archaeon from salt-fermented food. Int J Syst Evol Microbiol 59, 2359–2363.[CrossRef]
    [Google Scholar]
  21. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  22. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  23. Savage, K. N., Krumholz, L. R., Oren, A. & Elshahed, M. S. ( 2007; ). Haladaptatus paucihalophilus gen. nov., sp. nov., a halophilic archaeon isolated from a low-salt, sulfide-rich spring. Int J Syst Evol Microbiol 57, 19–24.[CrossRef]
    [Google Scholar]
  24. Sehgal, S. N. & Gibbons, N. E. ( 1960; ). Effect of some metal ions on the growth of Halobacterium cutirubrum. Can J Microbiol 6, 165–169.[CrossRef]
    [Google Scholar]
  25. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  26. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  27. Xin, H., Itoh, T., Zhou, P., Suzuki, K., Kamekura, M. & Nakase, T. ( 2000; ). Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xinjiang, China. Int J Syst Evol Microbiol 50, 1297–1303.[CrossRef]
    [Google Scholar]
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vol. , part 5, pp. 1187 - 1190

Thin-layer chromatograms of the polar lipids in type strains of species. (a) Two-dimensional TLC to detect phospholipids of strain D43 , stained with Zinzadze reagent. (b) One-dimensional TLC to detect glycolipids, stained with α-naphthol reagent. Lanes: 1, KCTC 4006 ; 2, sp. nov. D43 . GL, Unidentified glycolipid; PG, phosphatidylglycerol; PGP-Me, phosphatidylglycerol phosphate methyl ester. See main text for references for staining protocols.



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