1887

Abstract

A Gram-stain-positive, motile, rod-shaped bacterial strain, ISL-17, was isolated from a marine solar saltern of the Yellow Sea, Korea, and its taxonomic position was investigated by means of a polyphasic study. Strain ISL-17 grew optimally at pH 8.5–9.0, at 37 °C and in the presence of approximately 10 % (w/v) NaCl. It contained -diaminopimelic acid as the diagnostic diamino acid in the peptidoglycan, MK-7 as the predominant menaquinone and iso-C, anteiso-C and iso-C as the major fatty acids. The DNA G+C content was 48.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISL-17 fell within the genus , clustering with BH163 with a bootstrap resampling value of 100 %. Strain ISL-17 exhibited 98.2 % 16S rRNA gene sequence similarity to . BH163 and 95.8–96.5 % similarity to the type strains of the other species. The mean DNA–DNA relatedness value between strain ISL-17 and . KCTC 3916 was 19 %. The phenotypic properties of strain ISL-17, together with its phylogenetic and genetic distinctiveness, enable this strain to be differentiated from recognized species. On the basis of phenotypic, phylogenetic and genetic data, strain ISL-17 represents a novel species within the genus , for which the name sp. nov. is proposed; the type strain is ISL-17 (=KCTC 13258 =CCUG 56753).

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2010-02-01
2019-12-11
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