1887

Abstract

Gram-positive, non-spore-forming rods were isolated from a human osteo-articular sample (strain 7400942). Based on cellular morphology and the results of biochemical analysis, this strain was tentatively identified as a novel species of the genus . Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the bacterium was closely related to the type strain of (96.9 % 16S rRNA gene sequence similarity). A comparison of biochemical traits showed that strain 7400942 was distinct from in a number of characteristics, i.e. in contrast with , strain 7400942 was negative for nitrate reduction and for -galactosidase, -glucosidase and alanine arylamidase activities, it was positive for acid production from -acetylglucosamine, melezitose and glycogen, and it was negative for acid production from turanose. Matrix-assisted laser-desorption/ionization time-of-flight MS protein analysis confirmed that strain 7400942 represents a novel species, as scores obtained for its spectra were significant (>2.2) only with strain 7400942. On the basis of phenotypic data and phylogenetic inference, it is proposed that this strain should be designated sp. nov.; the type strain is strain 7400942 (=CSUR P35=CCUG 55928).

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2010-07-01
2020-01-21
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References

  1. Acevedo, F., Baudrand, R., Letelier, L. M. & Gaete, P. ( 2008; ). Actinomycosis: a great pretender. Case reports of unusual presentations and a review of the literature. Int J Infect Dis 12, 358–362.[CrossRef]
    [Google Scholar]
  2. An, D., Cai, S. & Dong, X. ( 2006; ). Actinomyces ruminicola sp. nov., isolated from cattle rumen. Int J Syst Evol Microbiol 56, 2043–2048.[CrossRef]
    [Google Scholar]
  3. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  4. Funke, G., von Graevenitz, A., Clarridge, J. E., III & Bernard, K. E. ( 1997; ). Clinical microbiology of coryneform bacteria. Clin Microbiol Rev 10, 125–159.
    [Google Scholar]
  5. Gouriet, F., Fenollar, F., Patrice, J. Y., Drancourt, M. & Raoult, D. ( 2005; ). Use of shell-vial cell culture assay for isolation of bacteria from clinical specimens: 13 years of experience. J Clin Microbiol 43, 4993–5002.[CrossRef]
    [Google Scholar]
  6. Hall, V. ( 2008; ). Actinomyces – gathering evidence of human colonization and infection. Anaerobe 14, 1–7.[CrossRef]
    [Google Scholar]
  7. Hall, V., Talbot, P. R., Stubbs, S. L. & Duerden, B. I. ( 2001; ). Identification of clinical isolates of Actinomyces species by amplified 16S ribosomal DNA restriction analysis. J Clin Microbiol 39, 3555–3562.[CrossRef]
    [Google Scholar]
  8. Hall, V., Collins, M. D., Hutson, R. A., Inganas, E., Falsen, E. & Duerden, B. I. ( 2003a; ). Actinomyces oricola sp. nov., from a human dental abscess. Int J Syst Evol Microbiol 53, 1515–1518.[CrossRef]
    [Google Scholar]
  9. Hall, V., Collins, M. D., Lawson, P. A., Falsen, E. & Duerden, B. I. ( 2003b; ). Actinomyces nasicola sp. nov., isolated from a human nose. Int J Syst Evol Microbiol 53, 1445–1448.[CrossRef]
    [Google Scholar]
  10. Hall, V., Collins, M. D., Lawson, P. A., Falsen, E. & Duerden, B. I. ( 2005; ). Actinomyces dentalis sp. nov., from a human dental abscess. Int J Syst Evol Microbiol 55, 427–431.[CrossRef]
    [Google Scholar]
  11. Kerttula, A. M., Carlson, P., Sarkonen, N., Hall, V. & Könönen, E. ( 2005; ). Enzymatic/biochemical analysis of Actinomyces with commercial test kits with an emphasis on newly described species. Anaerobe 11, 99–108.[CrossRef]
    [Google Scholar]
  12. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  13. Mellmann, A., Cloud, J., Maier, T., Keckevoet, U., Ramminger, I., Iwen, P., Dunn, J., Hall, G., Wilson, D. & other authors ( 2008; ). Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria. J Clin Microbiol 46, 1946–1954.[CrossRef]
    [Google Scholar]
  14. Miller, P. H., Wiggs, L. S. & Miller, J. M. ( 1995; ). Evaluation of API An-IDENT and RapID ANA II systems for identification of Actinomyces species from clinical specimens. J Clin Microbiol 33, 329–330.
    [Google Scholar]
  15. Pascual, C., Foster, G., Falsen, E., Bergström, K., Greko, C. & Collins, M. D. ( 1999; ). Actinomyces bowdenii sp. nov., isolated from canine and feline clinical specimens. Int J Syst Bacteriol 49, 1873–1877.[CrossRef]
    [Google Scholar]
  16. Rothschild, B., Naples, V. & Barbian, L. ( 2006; ). Bone manifestations of actinomycosis. Ann Diagn Pathol 10, 24–27.[CrossRef]
    [Google Scholar]
  17. Saitou, N. & Nei, M. ( 1987; ). The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  18. Santala, A. M., Sarkonen, N., Hall, V., Carlson, P., Jousimies-Somer, H. & Könönen, E. ( 2004; ). Evaluation of four commercial test systems for identification of Actinomyces and some closely related species. J Clin Microbiol 42, 418–420.[CrossRef]
    [Google Scholar]
  19. Song, Y. ( 2005; ). PCR-based diagnostics for anaerobic infections. Anaerobe 11, 79–91.[CrossRef]
    [Google Scholar]
  20. Stîngu, C. S., Rodloff, A. C., Jentsch, H., Schaumann, R. & Eschrich, K. ( 2008; ). Rapid identification of oral anaerobic bacteria cultivated from subgingival biofilm by MALDI-TOF-MS. Oral Microbiol Immunol 23, 372–376.[CrossRef]
    [Google Scholar]
  21. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  22. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J. ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703.
    [Google Scholar]
  23. Woo, P. C., Ng, K. H. L., Lau, S. K. P., Yip, K. T., Fung, A. M. Y., Leung, K. W., Tam, D. M. W., Que, T. L. & Yuen, K. Y. ( 2003; ). Usefulness of the MicroSeq 500 16S ribosomal DNA-based bacterial identification system for identification of clinically significant bacterial isolates with ambiguous biochemical profiles. J Clin Microbiol 41, 1996–2001.[CrossRef]
    [Google Scholar]
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