1887

Abstract

Gram-positive, non-spore-forming rods were isolated from a human osteo-articular sample (strain 7400942). Based on cellular morphology and the results of biochemical analysis, this strain was tentatively identified as a novel species of the genus . Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the bacterium was closely related to the type strain of (96.9 % 16S rRNA gene sequence similarity). A comparison of biochemical traits showed that strain 7400942 was distinct from in a number of characteristics, i.e. in contrast with , strain 7400942 was negative for nitrate reduction and for -galactosidase, -glucosidase and alanine arylamidase activities, it was positive for acid production from -acetylglucosamine, melezitose and glycogen, and it was negative for acid production from turanose. Matrix-assisted laser-desorption/ionization time-of-flight MS protein analysis confirmed that strain 7400942 represents a novel species, as scores obtained for its spectra were significant (>2.2) only with strain 7400942. On the basis of phenotypic data and phylogenetic inference, it is proposed that this strain should be designated sp. nov.; the type strain is strain 7400942 (=CSUR P35=CCUG 55928).

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2010-07-01
2021-03-01
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