@article{mbs:/content/journal/ijsem/10.1099/ijs.0.012880-0, author = "Sorokin, Dimitry Yu. and Kovaleva, Olga L. and Tourova, Tatjana P. and Muyzer, Gerard", title = "Thiohalobacter thiocyanaticus gen. nov., sp. nov., a moderately halophilic, sulfur-oxidizing gammaproteobacterium from hypersaline lakes, that utilizes thiocyanate", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2010", volume = "60", number = "2", pages = "444-450", doi = "https://doi.org/10.1099/ijs.0.012880-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.012880-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "SOB, sulfur-oxidizing bacteria", keywords = "COS, carbonyl sulfide", keywords = "RuBisCO, ribulose bisphosphate carboxylase/oxygenase", abstract = "A moderately halophilic, obligately chemolithoautotrophic, sulfur-oxidizing bacterium, designated strain HRh1T, was obtained from mixed sediment samples from hypersaline chloride–sulfate lakes in the Kulunda Steppe, in south-western Siberia (Russia), using aerobic enrichment culture at 1 M NaCl with thiocyanate as substrate. Cells of the isolate were short, non-motile rods with a Gram-negative type of cell wall. The bacterium was an obligate aerobe capable of chemolithoautotrophic growth with thiocyanate and thiosulfate. With thiosulfate, it grew at NaCl concentrations of 0.2–3.0 M (optimum 0.5 M) and at pH 6.3–8.0 (optimum pH 7.3–7.5). During growth on thiocyanate, cyanate was identified as an intermediate. The dominant cellular fatty acids were C16 : 0 and C18 : 1 ω7. Phylogenetic analysis based on 16S rRNA gene sequencing placed the isolate in the class Gammaproteobacteria as an independent lineage, with an unclassified marine sulfur-oxidizing bacterium as the closest culturable relative (93 % sequence similarity). A single cbbL gene (coding for the key enzyme of the Calvin–Benson cycle of autotrophic CO2 assimilation) with relatively low similarity to any homologous genes found in chemolithoautotrophs was detected in strain HRh1T. On the basis of our phenotypic and phylogenetic analysis, the halophilic isolate is proposed to represent a new genus and novel species, Thiohalobacter thiocyanaticus gen. nov., sp. nov. The type strain of Thiohalobacter thiocyanaticus is HRh1T (=DSM 21152T =UNIQEM U249T).", }