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Abstract

A novel actinomycete was isolated from soil of a rock surface collected from the peak of Darangshi Oreum (Small Mountain) in Jeju, Republic of Korea. Phylogenetic analyses based on 16S rRNA gene sequences demonstrated that strain DLS-44 belonged to the genus , with the type strains of (99.1 % sequence similarity) and (99.0 %) as the nearest phylogenetic relatives. Substrate mycelium was abundant, irregularly branched, twisted and vivid orange–yellow in colour. Aerial mycelium was not produced on most media tested. Finger-shaped sporangia and globose bodies were formed directly from the vegetative mycelium. The combination of morphological and chemotaxonomic characteristics supported assignment of the actinomycete to the genus . Strain DLS-44 could be distinguished clearly from all type strains of the genus based on its physiological properties (utilization of methyl --mannoside and glycerol, nitrate reduction and growth at 20 °C and pH 9.1) and some chemotaxonomic characteristics (absence of unsaturated fatty acids). DNA–DNA relatedness values between strain DLS-44 and its closest phylogenetic relatives were 12.2–14.8 % with DSM 43916 and 2.5–3.6 % with IMSNU 22055. On the basis of phenotypic, phylogenetic and DNA–DNA hybridization data, strain DLS-44 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain DLS-44 (=KCTC 19560 =DSM 45260).

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2010-06-01
2021-10-27
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