1887

Abstract

Four Gram-stain-positive, catalase-negative, coccoid-shaped isolates were obtained from the oral cavities of wild boars and characterized by phenotypic and phylogenetic studies. On the results of biochemical tests, the organisms were tentatively identified as a streptococcal species. Comparative 16S rRNA gene sequencing studies confirmed that the organisms are members of the genus , with subsp. ATCC 33398 as their closest phylogenetic relative (94.7 % similarity). DNA–DNA hybridization analysis showed that the isolates displayed less than 10 % relatedness to subsp. DSM 20561. From the phylogenetic and phenotypic evidence, the four isolates represent a novel species of the genus , for which the name sp. nov. (type strain NUM 1529 =JCM 15752 =DSM 21999) is proposed.

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2010-04-01
2020-09-30
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References

  1. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  2. Felsenstein J. 1985; Confidence limits of phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  3. Fukushima K., Motoda R., Ikeda T. 1981; Effects of exogenous insoluble glucan primer on insoluble glucan synthesis by Streptococcus mutans . J Dent Res 60:1707–1712 [CrossRef]
    [Google Scholar]
  4. Hamada S., Slade H. D. 1980; Biology, immunology, and cariogenicity of Streptococcus mutans . Microbiol Rev 44:331–384
    [Google Scholar]
  5. Hirasawa M., Takada K. 1994; Porphyromonas gingivicanis sp. nov. and Porphyromonas crevioricanis sp. nov., isolated from beagles. Int J Syst Bacteriol 44:637–640 [CrossRef]
    [Google Scholar]
  6. Hirasawa M., Kiyono H., Shiota T., Michalek S. M., McGhee J. R. 1980; Virulence of Streptococcus mutans : immunochemical characterization of a serotype g -defective mutant (C307). Infect Immun 27:697–699
    [Google Scholar]
  7. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  8. Loesche W. J. 1986; Role of Streptococcus mutans in human dental decay. Microbiol Rev 50:353–380
    [Google Scholar]
  9. Nakano K., Nomura R., Nakagawa I., Hamada S., Ooshima T. 2004; Demonstration of Streptococcus mutans with a cell wall polysaccharide specific to a new serotype, k , in the human oral cavity. J Clin Microbiol 42:198–202 [CrossRef]
    [Google Scholar]
  10. Rantz L. A., Randall E. 1955; Use of autoclaved extracts of hemolytic streptococci for serological grouping. Stanford Med Bull 13:290–291
    [Google Scholar]
  11. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  12. Shinozaki-Kuwahara N., Takada K., Hirasawa M. 2008; Sequence and phylogenetic analyses of novel glucosyltransferase gene of mutans streptococci isolated from pig oral cavity. J Microbiol 46:202–208 [CrossRef]
    [Google Scholar]
  13. Takada K., Hirasawa M. 2007; Streptococcus orisuis sp. nov., isolated from the pig oral cavity. Int J Syst Evol Microbiol 57:1272–1275 [CrossRef]
    [Google Scholar]
  14. Takada K., Hirasawa M. 2008; Streptococcus dentirousetti sp. nov., isolated from the oral cavity of bats. Int J Syst Evol Microbiol 58:160–163 [CrossRef]
    [Google Scholar]
  15. Takada K., Wyszomirska J., Shiota T. 1984; Serological characterization of Streptococcus mutans serotype polysaccharide g and its different molecular weight forms. Infect Immun 45:464–469
    [Google Scholar]
  16. Takada K., Igarashi M., Yamaguchi Y., Hirasawa M. 2008; New serotype of mutans streptococci isolated from pig oral cavity. Microbiol Immunol 52:64–68 [CrossRef]
    [Google Scholar]
  17. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  18. Van Handel E. 1967; Determination of fructose and fructose-yielding carbohydrates with cold anthrone. Anal Biochem 19:193–194 [CrossRef]
    [Google Scholar]
  19. Whiley R. A., Beighton D. 1998; Current classification of the oral streptococci. Oral Microbiol Immunol 13:195–216
    [Google Scholar]
  20. Yamaguchi Y. 2008; Cariogenicity of three kinds of mutans streptococci from pig oral cavity. Int J Oral Med Sci 7:67–71
    [Google Scholar]
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