1887

Abstract

A novel actinomycete, designated strain GUI 15, isolated from the root nodules of a plant was characterized taxonomically by using a polyphasic approach. The 16S rRNA gene sequence of strain GUI 15 showed highest similarity to TJ2-2 (98.7 %) and TT 97-42 (98.5 %). Phylogenetic analysis based on the gyrase B gene also supported the close relationship of these three strains, but indicated that strain GUI 15 should be assigned to the genus . Chemotaxonomic results confirmed the position of the isolate in the genus , but revealed differences at the species level. The novel strain could be distinguished from recognized species by using a combination of physiological and biochemical tests. Based on these observations, strain GUI 15 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GUI 15 (=DSM 45175=LMG 24546).

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2010-02-01
2020-01-18
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References

  1. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  2. Doetsch, R. N. ( 1981; ). Determinative methods of light microscopy. In Manual of Methods for General Bacteriology, pp. 21–33. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Washington, DC: American Society for Microbiology.
  3. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  4. Hasegawa, T., Takizawa, M. & Tanida, S. ( 1983; ). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef]
    [Google Scholar]
  5. Jones, K. L. ( 1949; ). Fresh isolates of actinomycetes in which the presence of sporogeneous aerial mycelia is a fluctuating characteristic. J Bacteriol 57, 141–146.
    [Google Scholar]
  6. Kasai, H., Tamura, T. & Harayama, S. ( 2000; ). Intrageneric relationships among Micromonospora species deduced from gyrB-based phylogeny and DNA relatedness. Int J Syst Evol Microbiol 50, 127–134.[CrossRef]
    [Google Scholar]
  7. Kawamoto, I., Yamamoto, M. & Nara, T. ( 1983; ). Micromonospora olivasterospora sp. nov. Int J Syst Bacteriol 33, 107–112.[CrossRef]
    [Google Scholar]
  8. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  9. Kroppenstedt, R. M. ( 1985; ). Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20), pp. 173–199. Edited by M. Goodfellow & D. E. Minnikin. New York: Academic Press.
  10. Lechevalier, M. P., De Bièvre, C. & Lechevalier, H. ( 1977; ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef]
    [Google Scholar]
  11. Mandel, M. & Marmur, J. ( 1968; ). Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B, 195–206.
    [Google Scholar]
  12. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  13. Ørskov, J. ( 1923; ). Investigations into the Morphology of the Ray Fungi. Copenhagen: Levin and Munksgaard.
  14. Rhuland, L. E., Work, E., Denman, R. F. & Hoare, D. S. ( 1955; ). The behavior of the isomers of α,ϵ-diaminopimelic acid on paper chromatograms. J Am Chem Soc 77, 4844–4846.[CrossRef]
    [Google Scholar]
  15. Richert, K., Brambilla, E. & Stackebrandt, E. ( 2007; ). The phylogenetic significance of peptidoglycan types: molecular analysis of the genera Microbacterium and Aureobacterium based upon sequence comparison of gyrB, rpoB, recA and ppk and 16S rRNA genes. Syst Appl Microbiol 30, 102–108.[CrossRef]
    [Google Scholar]
  16. Rivas, R., Sánchez, M., Trujillo, M. E., Zurdo-Piñeiro, J. L., Mateos, P. F., Martínez-Molina, E. & Velázquez, E. ( 2003; ). Xylanimonas cellulosilytica gen. nov., sp. nov., a xylanolytic bacterium isolated from a decayed tree (Ulmus nigra). Int J Syst Evol Microbiol 53, 99–103.[CrossRef]
    [Google Scholar]
  17. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  18. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  19. Schröder, K.-H., Naumann, L., Kroppenstedt, R. M. & Reischl, U. ( 1997; ). Mycobacterium hassiacum sp. nov., a new rapidly growing thermophilic mycobacterium. Int J Syst Bacteriol 47, 86–91.[CrossRef]
    [Google Scholar]
  20. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  21. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to the identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  22. Tamura, T., Hatano, K. & Suzuki, K. ( 2006; ). A new genus of the family Micromonosporaceae, Polymorphospora gen. nov., with description of Polymorphospora rubra sp. nov. Int J Syst Evol Microbiol 56, 1959–1964.[CrossRef]
    [Google Scholar]
  23. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  24. Thawai, C., Tanasupawat, S. & Kudo, T. ( 2008; ). Micromonospora pattaloongensis sp. nov., isolated from a Thai mangrove forest. Int J Syst Evol Microbiol 58, 1516–1521.[CrossRef]
    [Google Scholar]
  25. Trujillo, M. E., Fernández-Molinero, C., Velázquez, E., Kroppenstedt, R. M., Schumann, P., Mateos, P. F. & Martínez-Molina, E. ( 2005; ). Micromonospora mirobrigensis sp. nov. Int J Syst Evol Microbiol 55, 877–880.[CrossRef]
    [Google Scholar]
  26. Trujillo, M. E., Kroppenstedt, R. M., Schumann, P. & Martínez-Molina, E. ( 2006a; ). Kribbella lupini sp. nov., isolated from the roots of Lupinus angustifolius. Int J Syst Evol Microbiol 56, 407–411.[CrossRef]
    [Google Scholar]
  27. Trujillo, M. E., Kroppenstedt, R. M., Schumann, P., Carro, L. & Martínez-Molina, E. ( 2006b; ). Micromonospora coriariae sp. nov., isolated from root nodules of Coriaria myrtifolia. Int J Syst Evol Microbiol 56, 2381–2385.[CrossRef]
    [Google Scholar]
  28. Trujillo, M. E., Kroppenstedt, R. M., Fernández-Molinero, C., Schumann, P. & Martínez-Molina, E. ( 2007; ). Micromonospora lupini sp. nov. and Micromonospora saelicesensis sp. nov., isolated from root nodules of Lupinus angustifolius. Int J Syst Evol Microbiol 57, 2799–2804.[CrossRef]
    [Google Scholar]
  29. Vincent, J. M. ( 1970; ). The cultivation, isolation and maintenance of rhizobia. In A Manual for the Practical Study of the Root-Nodule Bacteria, pp. 1–13. Edited by J. M. Vincent. Oxford: Blackwell Scientific.
  30. Williams, S. T., Goodfellow, M., Alderson, G., Wellington, E. M. H., Sneath, P. H. A. & Sackin, M. J. ( 1983; ). Numerical classification of Streptomyces and related genera. J Gen Microbiol 129, 1743–1813.
    [Google Scholar]
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Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showing the relationship between strain GUI 15 , recognized species and other members of the family . [PDF](41 KB)

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Cultural characteristics of strain GUI 15 observed on various growth media. [PDF](10 KB)

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Fatty acid compositions of strain GUI 15 and TT 97-42 . [PDF](14 KB)

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