1887

Abstract

A novel, strictly aerobic, Gram-stain-negative, yellow-orange-pigmented, rod-shaped bacterium was isolated from abalone () under aquaculture in seawater off the Wando coast, Southern Korea, and subjected to a polyphasic taxonomic study. Cells of strain Haldis-1 were catalase- and oxidase-positive rods with flexirubin pigments. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Haldis-1 formed a distinct lineage within the family and could be distinguished from the related genera and . Strain Haldis-1 shared 16S rRNA gene sequence similarities of 92.5 and 92.4 % with UST040201-001 and RW262, respectively. The DNA G+C content was 38.1 mol% and the major respiratory quinone was MK-7. The predominant cellular fatty acids were iso-C (38.6 %), C 2-OH (20.3 %) and C (10.7 %). Growth was observed at 25–42 °C (optimum 30–37 °C) and at pH 6.5–9.5 (optimum pH 6.5–8.0). On the basis of polyphasic analysis of phenotypic, genotypic and phylogenetic data, strain Haldis-1 represents a novel genus and species within the family in the phylum , for which the name gen. nov., sp. nov. is proposed. The type strain is Haldis-1 (=KCTC 22610 =NBRC 105642).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.012674-0
2010-03-01
2020-01-17
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/3/510.html?itemId=/content/journal/ijsem/10.1099/ijs.0.012674-0&mimeType=html&fmt=ahah

References

  1. Bernardet, J. F., Nakagawa, Y. & Holmes, B. ( 2002; ). Proposed minimal standards for describing new taxa of thefamily Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef]
    [Google Scholar]
  2. Bowman, J. P., Mancuso Nichols, C. & Gibson, J. A. E. ( 2003; ). Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignavagen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp.nov., novel flavobacteria isolated from various polar habitats. Int J Syst Evol Microbiol 53, 1343–1355.[CrossRef]
    [Google Scholar]
  3. Fautz, E. & Reichenbach, H. ( 1980; ). A simple test for flexirubin-type pigments. FEMS MicrobiolLett 8, 87–91.
    [Google Scholar]
  4. Felsenstein, J. ( 2002; ). phylip (phylogeny inference package), version 3.6a. Distributedby the author. Department of Genome Sciences, University of Washington, Seattle,USA.
  5. Grossart, H. P., Levold, F., Allgaier, M., Simon, M. & Brinkhoff,T. ( 2005; ). Marine diatom species harbour distinctbacterial communities. Environ Microbiol 7, 860–873.[CrossRef]
    [Google Scholar]
  6. Jeon, C. O., Park, W., Ghiorse, W. C. & Madsen, E. L. ( 2004; ). Polaromonas naphthalenivorans sp. nov.,a naphthalene-degrading bacterium from naphthalene-contaminated sediment. Int J Syst Evol Microbiol 54, 93–97.[CrossRef]
    [Google Scholar]
  7. Komagata, K. & Suzuki, K. ( 1987; ).Lipid and cell-wall analysis in bacterial systematics. MethodsMicrobiol 19, 161–207.
    [Google Scholar]
  8. Lane, D. J. ( 1991; ). 16S/23S rRNAsequencing. In Nucleic Acid Techniques in Bacterial Systematics,pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester:Wiley.
  9. Lányí, B. ( 1987; ). Classicaland rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  10. Lau, K. W. K., Ng, C. Y. M., Ren, J., Lau, S. C. L., Qian, P.-Y.,Wong, P.-K., Lau, T. C. & Wu, M. ( 2005; ). Owenweeksia hongkongensis gen. nov., sp. nov., a novel marine bacteriumof the phylum ‘Bacteroidetes’. Int J Syst Evol Microbiol 55, 1051–1057.[CrossRef]
    [Google Scholar]
  11. Lau, K. W. K., Ren, J., Wai, N. L. M., Qian, P.-Y., Wong, P.-K. &Wu, M. ( 2006; ). Lishizhenia caseinilyticagen. nov., sp. nov., a marine bacterium of the phylum Bacteroidetes. Int J Syst Evol Microbiol 56, 2317–2322.[CrossRef]
    [Google Scholar]
  12. Leifson, E. ( 1963; ). Determination ofcarbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  13. O'Sullivan, L. A., Linna, J., Humphreys, G., Weightman,A. J. & Fry, J. C. ( 2005; ). Fluviicolor taffensis gen. nov., sp. nov., a novel freshwater bacterium of the family Cryomorphaceae in the phylum ‘Bacteroidetes’. Int J Syst Evol Microbiol 55, 2189–2194.[CrossRef]
    [Google Scholar]
  14. Pearson, W. R. & Lipman, D. J. ( 1988; ). Improved tools for biological sequence comparison. Proc NatlAcad Sci U S A 85, 2444–2448.
    [Google Scholar]
  15. Pinhassi, J., Sala, M. M., Havskum, H., Peters, F., Guadayol,O., Malits, A. & Marrase, C. ( 2004; ). Changes inbacterioplankton composition under different phytoplankton regimens. Appl Environ Microbiol 70, 6753–6766.[CrossRef]
    [Google Scholar]
  16. Reva, O. N., Vyunitskaya, V. A., Reznik, S. R., Kozachko, I.A. & Smirnov, V. V. ( 1995; ). Antibiotic susceptibilityas a taxonomic characteristic of the genus Bacillus. Int J Syst Bacteriol 45, 409–411.[CrossRef]
    [Google Scholar]
  17. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and MolecularBacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray,W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  18. Tamaoka, J. & Komagata, K. ( 1984; ).Determination of DNA base composition by reversed-phase high-performance liquidchromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  19. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivityof progressive multiple sequence alignment through sequence weighting, position-specificgap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.012674-0
Loading
/content/journal/ijsem/10.1099/ijs.0.012674-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error