1887

Abstract

A novel facultatively anaerobic strain, designated GPTSA 19, was isolated from a warm spring and characterized using a polyphasic approach. The strain behaved as Gram-negative in the Gram staining procedure but showed a Gram-positive reaction in the aminopeptidase test. The novel strain was a mesophilic rod with ellipsoidal endospores. On the basis of 16S rRNA gene sequence analysis, the strain showed closest similarity (96.0 %) with MC10. The gene sequence similarity of the novel strain with other species of the genus was <95.8 %. The novel strain also had PAEN 515F and 682F signature sequence stretches in the 16S rRNA gene that are usually found in most species of the genus . The strain possessed anteiso-C as the major fatty acid and MK-7 as the predominant menaquinone. Polar lipids included diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), six unknown phospholipids (PLs), one aminophospholipid (PN), three glycolipids (GLs), two aminolipids (ALs), one aminophosphoglycolipid (APGL) and three unknown lipids (ULs). The polar lipid profile of the novel strain, especially as regards ALs, GLs and PLs, distinguished it from the recognized type species of the genus , , as well as from its closest relative . Based on phenotypic and chemotaxonomic characteristics and analysis of the 16S rRNA gene sequence, the new strain merits the rank of a novel genus for which the name gen. nov. is proposed. The type species of the new genus is gen. nov., sp. nov. with the type strain GPTSA 19 (=MTCC 7155=DSM 17643).

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2010-02-01
2020-01-27
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Cellular fatty acid contents of strain GPTSA 19 and closely related species of the genus and of . [ PDF] 50 KB

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Comparative total polar lipid profile of , and strain GPTSA19 . DPG, diphosphatidylglycerol; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; GL, glycolipid; UL, unknown lipid; PN, aminophospholipid; APGL, aminophosphoglycolipid; AL, aminolipid; PL, phospholipid. Numbering of polar lipids is according to the convention adopted by Kämpfer , 2006.

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