1887

Abstract

Three strains, KV-810, KV-811 and KV-816, were isolated from mangrove soil from a southern island in Japan on media supplemented with ascorbic acid or rutin. These strains contained -ornithine as the diagnostic diamino acid in the cell-wall peptidoglycan and DMK-9(H) as the predominant menaquinone. The G+C content of the DNA was 70–72 mol%. These characteristics in combination with 16S rRNA gene sequence analysis revealed that the novel strains belonged to the genus . The DNA–DNA hybridization values showed that the three new strains belonged to the same species, a novel species of the genus Therefore strains KV-810, KV-811 and KV-816 are proposed as representing a novel species, sp. nov. The type strain is KV-810 (=DSM 22060=NBRC 105323).

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2010-05-01
2020-01-24
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References

  1. Collins, M. D., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  2. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  3. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–379.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  5. Finster, K. W., Herbert, R. A., Kjeldsen, K. U., Schumann, P. & Lomstein, B. A. ( 2009; ). Demequina lutea sp. nov., isolated from a high Arctic permafrost soil. Int J Syst Evol Microbiol 59, 649–653.[CrossRef]
    [Google Scholar]
  6. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef]
    [Google Scholar]
  7. Kawamoto, I., Oka, T. & Nara, T. ( 1981; ). Cell wall composition of Micromonospora olivoasterospora, Micromonospora sagamiensis, and related organisms. J Bacteriol 146, 527–534.
    [Google Scholar]
  8. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  9. Matsumoto, A., Yamada, M., Ōmura, S. & Takahashi, Y. ( 2008; ). Microterricola viridarii gen. nov., sp. nov., a new member of the family Microbacteriaceae. Int J Syst Evol Microbiol 58, 1019–1023.[CrossRef]
    [Google Scholar]
  10. Minnikin, D. E., Patel, P. V., Alshamaony, L. & Goodfellow, M. ( 1977; ). Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27, 104–117.[CrossRef]
    [Google Scholar]
  11. Saito, H. & Miura, K. ( 1963; ). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72, 619–629.[CrossRef]
    [Google Scholar]
  12. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  13. Takahashi, Y., Katoh, S., Shikura, N., Tomoda, H. & Ōmura, S. ( 2003; ). Superoxide dismutase produced by soil bacteria increases bacterial colony growth from soil samples. J Gen Appl Microbiol 49, 263–266.[CrossRef]
    [Google Scholar]
  14. Takahashi, Y., Matsumoto, A., Morisaki, K. & Ōmura, S. ( 2006; ). Patulibacter minatonensis gen. nov., sp. nov., a novel actinobacterium isolated using an agar medium supplemented with superoxide dismutase, and proposal of Patulibacteraceae fam. nov. Int J Syst Evol Microbiol 56, 401–406.[CrossRef]
    [Google Scholar]
  15. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  16. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  17. Tomiyasu, I. ( 1982; ). Mycolic acid composition and thermally adaptative changes in Nocardia asteroides. J Bacteriol 151, 828–837.
    [Google Scholar]
  18. Uchida, K. & Aida, K. ( 1977; ). Acyl type of bacterial cell wall: its simple identification by colorimetric method. J Gen Appl Microbiol 23, 249–260.[CrossRef]
    [Google Scholar]
  19. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  20. Yi, H., Schumann, P. & Chun, J. ( 2007; ). Demequina aestuarii gen. nov., sp. nov., a novel actinomycete of the suborder Micrococcineae, and reclassification of Cellulomonas fermentans Bagnara et al. 1985 as Actinotalea fermentans gen. nov., comb. nov. Int J Syst Evol Microbiol 57, 151–156.[CrossRef]
    [Google Scholar]
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vol. , part 5, pp. 1206 - 1209

Scanning electron micrographs of cells of strain KV-810 (a) and strain KV-816 (b) cultured on TSY agar for 3 days at 27°C. Bars, 1 µm.

DNA–DNA relatedness values between JC2054 and strains KV-810 , KV-811 and KV-816. Data were obtained from this study.



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