1887

Abstract

A Gram-stain-negative, motile, rod-shaped, cellulose-degrading bacterial strain, BIO-TAS4-2, which belongs to the , was isolated from forest soil from Naejang Mountain, Korea, and its taxonomic position was investigated by using a polyphasic study. Strain BIO-TAS4-2 grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 0–1.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain BIO-TAS4-2 clustered with members of the genera , and of the family , with which it exhibited 16S rRNA gene sequence similarities of 93.5–94.2 %. Strain BIO-TAS4-2 contained Q-8 as the predominant ubiquinone and summed feature 3 (C 7 and/or iso-C 2-OH) and C as the major fatty acids. The DNA G+C content was 63.8 mol%. Strain BIO-TAS4-2 could be differentiated from members of phylogenetically related genera by differences in fatty acid composition, DNA G+C content and some phenotypic properties. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain BIO-TAS4-2 is considered to represent a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed, with BIO-TAS4-2 (=KCTC 22633=CCUG 57610) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.012591-0
2010-03-01
2021-04-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/3/615.html?itemId=/content/journal/ijsem/10.1099/ijs.0.012591-0&mimeType=html&fmt=ahah

References

  1. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  2. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376
    [Google Scholar]
  3. Felsenstein J. 1993 phylip (phylogeny inference package) version 3.5. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  4. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  5. Kluge A. G., Farris J. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32
    [Google Scholar]
  6. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  7. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  8. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  9. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  10. Stackebrandt E., Lang E., Cousin S., Päuker O., Brambilla E., Kroppenstedt R., Lünsdorf H. 2007; Deefgea rivuli gen. nov., sp. nov. a member of the class Betaproteobacteria . Int J Syst Evol Microbiol 57:639–645 [CrossRef]
    [Google Scholar]
  11. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  12. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  13. Weon H.-Y., Kim B.-Y., Yoo S.-H., Joa J.-H., Kwon S.-W., Kim W.-G. 2007 Andreprevotia chitinilytica gen. nov., sp. nov., isolated from forest soil from Halla Mountain, Jeju Island, Korea. Int J Syst Evol Microbiol 57, 1572–1575 [CrossRef]
  14. Yang H.-C., Im W.-T., An D.-S., Park W.-S., Kim I. S., Lee S.-T. 2005; Silvimonas terrae gen. nov., sp. nov. a novel chitin-degrading facultative anaerobe belonging to the ‘ Betaproteobacteria ’. Int J Syst Evol Microbiol 55:2329–2332
    [Google Scholar]
  15. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  16. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
  17. Yoon J.-H., Kang K. H., Park Y.-H. 2003; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.012591-0
Loading
/content/journal/ijsem/10.1099/ijs.0.012591-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error