A Gram-staining-negative, rod-shaped, oxidase-positive, aerobic, non-motile and yellow-pigmented bacterial strain containing flexirubin type pigments, designated H1T, was isolated from raw cow'smilk in Israel.16S rRNA gene sequence analysis indicated that the isolate should be placed in the genus Epilithonimonas (family Flavobacteriaceae, phylum Bacteroidetes). The level of 16S rRNA gene sequence similarity between strain H1T and the type strain of Epilithonimonas tenax was 97.6 %. Strain H1T grew at 5–33 °C and with 0–2.0 % NaCl. The dominant cellular fatty acids of strain H1T were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1ω7c), and the DNA G+C content was 38.0 mol%. On the basis of phenotypic properties and phylogenetic distinctiveness, the milk isolate is classified as a new species in the genus Epilithonimonas, for which the name Epilithonimonas lactis sp. nov. (type strain H1T =LMG 24401T =DSM 19921T) is proposed.
AltschulS. F.,
MaddenT. L.,
SchäfferA. A.,
ZhangJ.,
ZhangZ.,
MillerW.,
LipmanD. J.1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402[CrossRef]
Ben-Ze'evI. S.,
LevyE.,
EilamT.,
AniksterY.2005; Whole-cell fatty acid profiles - a tool for species and subspecies classification in the Puccinia recondita complex. J Plant Pathol 87:187–197
BernardetJ.-F.,
NakagawaY.,
HolmesB.2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070[CrossRef]
ChunJ.,
LeeJ.-H.,
JungY.,
KimM.,
KimS.,
KimB. K.,
LimY. W.2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261[CrossRef]
FelskeA.,
RheimsH.,
WolterinkA.,
StackebrandtE.,
AkkermansA. D.1997; Ribosome analysis reveals prominent activity of an uncultured member of the class Actinobacteria in grassland soils. Microbiology 143:2983–2989[CrossRef]
Hantsis-ZacharovE.,
HalpernM.2007; Psychrotrophic bacterial communities in raw milk and their proteolytic and lipolytic traits. Appl Environ Microbiol 73:7162–7168[CrossRef]
MesbahM.,
PremachandranU.,
WhitmanW. B.1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167[CrossRef]
O'SullivanL. A.,
RinnaJ.,
HumphreysG.,
WeightmanA. J.,
FryJ. C.2006; Culturable phylogenetic diversity of the phylum ‘ Bacteroidetes ’ from river epilithon and coastal water and description of novel members of the family Flavobacteriaceae : Epilithonimonas tenax gen.nov., sp. nov. and Persicivirga xylanidelens gen. nov., sp. nov. Int J Syst Evol Microbiol 56:169–180[CrossRef]
WilsonK.1987; Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology pp.2.4.1–2.4.5 Edited by
AusubelF. M.,
BrentR.,
KingstonR. E.,
MooreD. D.,
SeidmanJ. G.,
SmithJ. A.,
StruhlK.
New York: Greene Publishing & Wiley-Interscience;
YoungC. C.,
KämpferP.,
ShenF. T.,
LaiW. A.,
ArunA. B.2005; Chryseobacterium formosense sp. nov., isolated from the rhizosphere of Lactuca sativa L. (garden lettuce). Int J Syst Evol Microbiol 55:423–426[CrossRef]