1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 60, Issue 6

sp. nov., an anaerobic, thermophilic, heterotrophic bacterium isolated from a hot spring Free

Abstract

An anaerobic thermophilic bacterium, strain K67, was isolated from a terrestrial hot spring of Uzon Caldera, Kamchatka Peninsula. Analysis of the 16S rRNA gene sequence revealed that the novel isolate belongs to the genus , with 95 % 16S rRNA gene sequence similarity to subsp. SEBR 7858, suggesting that it represents a novel species of the genus . Strain K67 was characterized as an obligate anaerobe, a thermophile (growth at 50–75 °С; optimum 68–70 °C), a neutrophile (growth at pH 4.8–8.0; optimum pH 6.8) and an obligate organotroph (growth by fermentation of various sugars, peptides and polysaccharides). Major fermentation products were acetate, H and CO; ethanol, lactate and -alanine were formed in smaller amounts. Thiosulfate stimulated growth and was reduced to hydrogen sulfide. Nitrate, sulfate, sulfite and elemental sulfur were not reduced and did not stimulate growth. Thus, according to the strain's phylogenetic position and phenotypic novelties (lower upper limit of temperature range for growth, the ability to grow on arabinose, the inability to reduce elemental sulfur and the formation of alanine as a minor fermentation product), the novel species sp. nov. is proposed, with the type strain K67 (=DSM 18923 =VKM В-2408).

  • Published Online:
SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.012328-0
2010-06-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/6/1372.html?itemId=/content/journal/ijsem/10.1099/ijs.0.012328-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
     [Google Scholar]
  2. Bonch-Osmolovskaya E. A., Sokolova T. G., Kostrikina N. A., Zavarzin G. A. 1990; Desulfurella acetovorans gen. nov., sp. nov. – a new thermophilic sulfur-reducing eubacterium. Arch Microbiol 153:151–155 [CrossRef]
     [Google Scholar]
  3. Fardeau M.-L., Magot M., Patel B. K. C., Thomas P., Garcia J.-L., Ollivier B. 2000; Thermoanaerobacter subterraneus sp. nov., a novel thermophile isolated from oilfield water. Int J Syst Evol Microbiol 50:2141–2149 [CrossRef]
     [Google Scholar]
  4. Fardeau M.-L., Salinas M. B., L'Haridon S., Jeanthon C., Verhé F., Cayol J.-L., Patel B. K. C., Garcia J.-L., Ollivier B. 2004; Isolation from oil reservoirs of novel thermophilic anaerobes phylogenetically related to Thermoanaerobacter subterraneus : reassignment of T. subterraneus , Thermoanaerobacter yonseiensis , Thermoanaerobacter tengcongensis and Carboxydibrachium pacificum to Caldanaerobacter subterraneus gen. nov., sp. nov., comb. nov. as four novel subspecies. Int J Syst Evol Microbiol 54:467–474 [CrossRef]
     [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
     [Google Scholar]
  6. Kevbrin V. V., Zavarzin G. A. 1992; The influence of sulfur compounds on the growth of halophilic homoacetic bacterium Acetohalobium arabaticum . Microbiology (English translation of Mikrobiologiia) 61:812–817
     [Google Scholar]
  7. Kim B.-C., Grote R., Lee D.-W., Antranikian G., Pyun Y.-R. 2001; Thermoanaerobacter yonseiensis sp. nov., a novel extremely thermophilic, xylose-utilizing bacterium that grows at up to 85 °C. Int J Syst Evol Microbiol 51:1539–1548
     [Google Scholar]
  8. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
     [Google Scholar]
  9. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
     [Google Scholar]
  10. Miroshnichenko M. L., Gongadze G. A., Lysenko A. M., Bonch-Osmolovskaya E. A. 1994; Desulfurella multipotens sp. nov., a new sulfur-respiring thermophilic eubacterium from Raoul Island (Kermadec archipelago. New Zealand). Arch Microbiol 161:88–93
     [Google Scholar]
  11. Moore S., Stein W. H. 1963; Chromatographic determination of amino acids by the use of automatic recording equipment. Methods Enzymol 6:819–831
     [Google Scholar]
  12. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
     [Google Scholar]
  13. Sokolova T. G., Gonzalez J. M., Kostrikina N. A., Chernyh N. A., Tourova T. P., Kato C., Bonch-Osmolovskaya E. A., Robb F. T. 2001; Carboxydobrachium pacificum gen. nov., sp. nov., a new anaerobic, thermophilic, CO-utilizing marine bacterium from Okinawa Trough. Int J Syst Evol Microbiol 51:141–149
     [Google Scholar]
  14. Subbotina I. V., Chernyh N. A., Sokolova T. G., Kublanov I. V., Bonch-Osmolovskaya E. A., Lebedinsky A. V. 2003; Oligonucleotide probes for the detection of the representatives of genus Thermoanaerobacter . Microbiology (English translation of Mikrobiologiia ) 72:331–339
     [Google Scholar]
  15. Tamura K., Nei M., Kumar S. 2004; Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci U S A 101:11030–11035 [CrossRef]
     [Google Scholar]
  16. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
     [Google Scholar]
  17. Wolin E. A., Wolin M. J., Wolfe R. S. 1963; Formation of methane by bacterial extracts. J Biol Chem 238:2882–2886
     [Google Scholar]
  18. Xue Y., Xu Y., Liu Y., Ma Y., Zhou P. 2001; Thermoanaerobacter tengcongensis sp. nov., a novel anaerobic, saccharolytic, thermophilic bacterium isolated from a hot spring in Tengcong, China. Int J Syst Evol Microbiol 51:1335–1341
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.012328-0
Loading
Caldanaerobacter uzonensis sp. nov., an anaerobic, thermophilic, heterotrophic bacterium isolated from a hot spring
Int J Syst Evol Microbiol 60, 1372 (2010); https://doi.org/10.1099/ijs.0.012328-0
/content/journal/ijsem/10.1099/ijs.0.012328-0
/content/journal/ijsem/10.1099/ijs.0.012328-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error