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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 60, Issue 6

sp. nov., an anaerobic, thermophilic, heterotrophic bacterium isolated from a hot spring Free

Abstract

An anaerobic thermophilic bacterium, strain K67, was isolated from a terrestrial hot spring of Uzon Caldera, Kamchatka Peninsula. Analysis of the 16S rRNA gene sequence revealed that the novel isolate belongs to the genus , with 95 % 16S rRNA gene sequence similarity to subsp. SEBR 7858, suggesting that it represents a novel species of the genus . Strain K67 was characterized as an obligate anaerobe, a thermophile (growth at 50–75 °С; optimum 68–70 °C), a neutrophile (growth at pH 4.8–8.0; optimum pH 6.8) and an obligate organotroph (growth by fermentation of various sugars, peptides and polysaccharides). Major fermentation products were acetate, H and CO; ethanol, lactate and -alanine were formed in smaller amounts. Thiosulfate stimulated growth and was reduced to hydrogen sulfide. Nitrate, sulfate, sulfite and elemental sulfur were not reduced and did not stimulate growth. Thus, according to the strain's phylogenetic position and phenotypic novelties (lower upper limit of temperature range for growth, the ability to grow on arabinose, the inability to reduce elemental sulfur and the formation of alanine as a minor fermentation product), the novel species sp. nov. is proposed, with the type strain K67 (=DSM 18923 =VKM В-2408).

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2010-06-01
2024-12-09
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Caldanaerobacter uzonensis sp. nov., an anaerobic, thermophilic, heterotrophic bacterium isolated from a hot spring
Int J Syst Evol Microbiol 60, 1372 (2010); https://doi.org/10.1099/ijs.0.012328-0
/content/journal/ijsem/10.1099/ijs.0.012328-0
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