1887

Abstract

A Gram-positive, aerobic, non-motile and coccoid actinobacterium, designated P31, was isolated from a traditional, fermented seafood. The strain was catalase-positive and oxidase-negative. Cells grew in the presence of 0–15.0 % (w/v) NaCl, and at pH 5–10 and 15–37 °C. Major cellular fatty acids were anteiso-C, anteiso-C and iso-C. Strain P31 contained MK-7 as the predominant menaquinone. The DNA G+C content of the genomic DNA of strain P31 was 65.2 mol%. A phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain P31 was most closely related to DSM 20032, with 96.9 % similarity, and these two strains clustered together in constructed phylogenetic trees. The DNA–DNA hybridization value between strain P31 and DSM 20032 was 21.1 %. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, it is suggested that strain P31 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is P31 (=KCTC 19595=JCM 15915).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.012310-0
2010-01-01
2021-03-03
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/1/140.html?itemId=/content/journal/ijsem/10.1099/ijs.0.012310-0&mimeType=html&fmt=ahah

References

  1. Gonzalez J. M., Saiz-Jimenez C. 2002; A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4:770–773 [CrossRef]
    [Google Scholar]
  2. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca , Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63 [CrossRef]
    [Google Scholar]
  3. Hiraishi A., Ueda Y., Ishihara J., Mori T. 1996; Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42:457–469 [CrossRef]
    [Google Scholar]
  4. Kim S. B., Nedashkovskaya O. I., Mikhailov V. V., Han S. K., Kim K.-O., Rhee M.-S., Bae K. S. 2004; Kocuria marina sp. nov., a novel actinobacterium isolated from marine sediment. Int J Syst Evol Microbiol 54:1617–1620 [CrossRef]
    [Google Scholar]
  5. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  6. Kluge A. G., Farris J. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [CrossRef]
    [Google Scholar]
  7. Kovacs G., Burghardt J., Pradella S., Schumann P., Stackebrandt E., Marialigeti K. 1999; Kocuria palustris sp. nov. and Kocuria rhizophila sp. nov., isolated from the rhizoplane of the narrow-leaved cattail ( Typha angustifolia . Int J Syst Bacteriol 49:167–173 [CrossRef]
    [Google Scholar]
  8. Li W.-J., Zhang Y.-Q., Schumann P., Chen H.-H., Hozzein W. N., Tian X.-P., Xu L.-H., Jiang C.-L. 2006; Kocuria aegyptia sp. nov., a novel actinobacterium isolated from a saline, alkaline desert soil in Egypt. Int J Syst Evol Microbiol 56:733–737 [CrossRef]
    [Google Scholar]
  9. Liu Z.-P., Wu J.-F., Liu Z.-H., Liu S.-J. 2006; Pseudonocardia ammonioxydans sp. nov., isolated from coastal sediment. Int J Syst Evol Microbiol 56:555–558 [CrossRef]
    [Google Scholar]
  10. Mayilraj S., Kroppenstedt R. M., Suresh K., Saini H. S. 2006; Kocuria himachalensis sp. nov., an actinobacterium isolated from the Indian Himalayas. Int J Syst Evol Microbiol 56:1971–1975 [CrossRef]
    [Google Scholar]
  11. Rainey F. A., Nobre M. F., Schumann P., Stackebrandt E., Da Costa M. S. 1997; Phylogenetic diversity of the deinococci as determined by 16S ribosomal DNA sequence comparison. Int J Syst Bacteriol 47:510–514 [CrossRef]
    [Google Scholar]
  12. Reddy G. S. N., Prakash J. S. S., Prabahar V., Matsumoto G. I., Stackebrandt E., Shivaji S. 2003; Kocuria polaris sp. nov., an orange-pigmented psychrophilic bacterium isolated from an Antarctic cyanobacterial mat sample. Int J Syst Evol Microbiol 53:183–187 [CrossRef]
    [Google Scholar]
  13. Reichert K., Lipski A., Pradella S., Stackebrandt E., Altendorf K. 1998; Pseudonocardia asaccharolytica sp. nov. and Pseudonocardia sulfidoxydans sp. nov., two new dimethyl disulfide-degrading actinomycetes and emended description of the genus Pseudonocardia . Int J Syst Bacteriol 48:441–449 [CrossRef]
    [Google Scholar]
  14. Roh S. W., Sung Y., Nam Y.-D., Chang H.-W., Kim K.-H., Yoon J.-H., Jeon C. O., Oh H.-M., Bae J.-W. 2008; Arthrobacter soli sp. nov., a novel bacterium isolated from wastewater reservoir sediment. J Microbiol 46:40–44 [CrossRef]
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  16. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  18. Seo Y. B., Kim D.-E., Kim G.-D., Kim H.-W., Nam S.-W., Kim Y. T., Lee J. H. 2009; Kocuria gwangalliensis sp. nov., an actinobacterium isolated from seawater. Int J Syst Evol Microbiol 59:2769–2772 [CrossRef]
    [Google Scholar]
  19. Stackebrandt E., Koch C., Gvozdiak O., Schumann P. 1995; Taxonomic dissection of the genus Micrococcus : Kocuria gen.nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend. Int J Syst Bacteriol 45:682–692 [CrossRef]
    [Google Scholar]
  20. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  21. Tang S.-K., Wang Y., Lou K., Mao P.-H., Xu L.-H., Jiang C.-L., Kim C.-J., Li W.-J. 2009; Kocuria halotolerans sp. nov., a novel actinobacterium isolated from a saline soil in China. Int J Syst Evol Microbiol 59:1316–1320 [CrossRef]
    [Google Scholar]
  22. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  23. Tittsler R. P., Sandholzer L. A. 1936; The use of semi-solid agar for the detection of bacterial motility. J Bacteriol 31:575–580
    [Google Scholar]
  24. Tvrzová L., Schumann P., Sedlácek I., Pácová Z., Spröer C., Verbarg S., Kroppenstedt R. M. 2005; Reclassification of strain CCM 132, previously classified as Kocuria varians , as Kocuria carniphila sp. nov. Int J Syst Evol Microbiol 55:139–142 [CrossRef]
    [Google Scholar]
  25. Zhou G., Luo X., Tang Y., Zhang L., Yang Q., Qio Y., Fang C. 2008 Kocuria flava sp. nov. and Kocuria turfanensis sp. nov., airborne actinobacteria isolated from Xinjiang, China. Int J Syst Evol Microbiol 58, 1304–1307 [CrossRef]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.012310-0
Loading
/content/journal/ijsem/10.1099/ijs.0.012310-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error