1887

Abstract

A novel endophytic actinomycete, strain GMKU 931, was isolated from the root of a wattle tree, A. Cunn. ex Benth., collected at Kasetsart University, Bangkok, Thailand. Strain GMKU 931 produced short spiral chains of smooth-surfaced spores on the aerial mycelium. Lysine and -diaminopimelic acid were present in the cell-wall peptidoglycan. Whole-cell hydrolysates contained galactose, madurose and mannose. The predominant menaquinones were MK-9(H) and MK-9(H). The major fatty acids were iso-C and iso-C. The major phospholipids were phosphatidylinositol and phosphatidylglycerol. A phylogenetic analysis based on 16S rRNA gene sequences suggested that strain GMKU 931 forms a distinct phyletic line within the recently proposed genus . The significant differences in phenotypic and genotypic data indicate that strain GMKU 931 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GMKU 931 (=BCC 28622 =NBRC 104354 =NRRL B-24610).

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2010-03-01
2024-03-29
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References

  1. Becker B., Lechevalier M. P., Lechevalier H. A. 1965; Chemical composition of cell-wall preparations from strains of various form-genera of aerobic actinomycetes. Appl Microbiol 13:236–243
    [Google Scholar]
  2. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  5. Goodfellow M. 1989; Maduromycetes. In Bergey's Manual of Systematic Bacteriology vol 4 pp 2509–2551 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  6. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca , Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63
    [Google Scholar]
  7. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [CrossRef]
    [Google Scholar]
  8. Hasegawa S., Meguro A., Shimizu M., Nishimura T., Kunoh H. 2006; Endophytic actinomycetes and their interactions with host plants. Actinomycetologica 20:72–81 [CrossRef]
    [Google Scholar]
  9. Hobbs G., Frazer C. M., Gardner D. C. J., Cullum J. A., Oliver S. G. 1989; Dispersed growth of Streptomyces in liquid culture. Appl Microbiol Biotechnol 31:272–277
    [Google Scholar]
  10. Kieser Y., Bibb M. J., Buttner M. J., Chater K. F., Hopwood D. A. 2000 Practical Streptomyces Genetics Norwich: The John Innes Foundation;
    [Google Scholar]
  11. Kroppenstedt R. M., Goodfellow M. 1992; The family Thermomonosporaceae . In The Prokaryotes , 2nd edn. pp 1085–1114 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
    [Google Scholar]
  12. Kunoh H. 2002; Endophytic actinomycetes: attractive biocontrol agents. J Gen Plant Pathol 68:249–252 [CrossRef]
    [Google Scholar]
  13. Küster E., Williams S. T. 1964; Media for the isolation of streptomycetes: starch casein medium. Nature 202:928–929 [CrossRef]
    [Google Scholar]
  14. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. other authors 2007; clustal w and clustal x version 2.0. Bioinformatics 23:2947–2948 [CrossRef]
    [Google Scholar]
  15. Lechevalier M. P., Lechevalier H. A. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [CrossRef]
    [Google Scholar]
  16. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  17. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  18. Nonomura H., Ohara Y. et al. 1971; Distribution of actinomycetes in soil. XI. Some new species of the genus Actinomadura Lechevalier et al.. J Ferment Technol 49:904–912
    [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  20. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  21. Tajima K., Takahashi Y., Seino A., Iwai Y., Ōmura S. 2001; Description of two novel species of the genus Kitasatospora Ōmura et al . 1982, Kitasatospora cineracea sp. nov. and Kitasatospora niigatensis sp. nov. Int J Syst Evol Microbiol 51:1765–1771 [CrossRef]
    [Google Scholar]
  22. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  23. Tamaoka J., Katayara-Fujimura Y., Kuraishi H. 1983; Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54:31–36 [CrossRef]
    [Google Scholar]
  24. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  25. Tamura T., Ishida Y., Nozawa Y., Otogturo M., Suzuki K. 2009 Transfer of Actinomadura spadix Nonomura and Ohara 1971 to Actinoallomurus spadix gen. nov., comb. nov., and description of Actinoallomurus amamiensis sp.nov., Actinoallomurus caesius sp. nov., Actinoallomurus coprocola sp. nov.,Actinoallomurus fulvus sp. nov., Actinoallomurus iriomotensis sp. nov., Actinoallomurus luridus sp. nov., Actinoallomurus purpureus sp.nov. and Actinoallomurus yoronensis sp. nov. Int J Syst Evol Microbiol 591867–1874 [CrossRef]
  26. Tomiyasu I. 1982; Mycolic acid composition and thermally adaptative changes in Nocardia asteroides . J Bacteriol 151:828–837
    [Google Scholar]
  27. Uchida K., Aida K. 1984; An improved method for the glycolate test for simple identification of acyl type of bacterial cell walls. J Gen Appl Microbiol 30:131–134 [CrossRef]
    [Google Scholar]
  28. Zhang Z., Wang Y., Ruan J. 1998; Reclassification of Thermomonospora and Microtetraspora . Int J Syst Bacteriol 48:411–422 [CrossRef]
    [Google Scholar]
  29. Zhang Z., Kudo T., Nakajima Y., Wang Y. 2001; Clarification of the relationship between the members of the family Thermomonosporaceae on the basis of 16S rDNA, 16S–23S rRNA internal transcribed spacer and 23S rDNA sequences and chemotaxonomic analyses. Int J Syst Evol Microbiol 51:373–383
    [Google Scholar]
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