An actinomycete strain, ID05-A0528T, was isolated using the SDS-yeast extract pre-treatment method from soil under mahogany (Swietenia mahogani) trees in West Timor, Indonesia, and was examined by using a polyphasic taxonomic approach. Chemotaxonomic and phylogenetic characterizations demonstrated that the novel strain belongs to the genus Dietzia. 16S rRNA gene sequencing studies showed that the strain was related to Dietzia cinnamea (97.2 %). Results of phenotypic and phylogenetic analyses determined that strain ID05-A0528T is different from the known species of the genus Dietzia. It is proposed that the isolate should be classified as a representative of a novel species of the genus Dietzia, with the name Dietzia timorensis sp. nov. The type strain is ID05-A0528T (=BTCC B-560T =NBRC 104184T).
DuckworthA. W.,
GrantS.,
GrantW. D.,
JonesB. E.,
MeijerD.1998; Dietzia natronolimnaios sp. nov., a new member of the genus Dietzia isolated from an east African soda lake. Extremophiles 2:359–366[CrossRef]
GerhardtP.,
MurrayR. G. E.,
CostilowR. N.,
NesterE. W.,
WoodW. A.,
KriegN. R.,
PhillipsG. B.
(editors) 1981Manual of Methods for General Bacteriology Washington, DC: American Society for Microbiology;
GordonR. E.,
BarnettD. A.,
HanderhanJ. E.,
PangC. H.-N.1974; Nocardia coeliaca , Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63[CrossRef]
HatanoK.,
NishiiT.,
KasaiH.2003 Taxonomic re-evaluation of whorl-forming Streptomyces (formerly Streptoverticillium ) species by using phenotypes, DNA–DNA hybridization and sequences of gyrB , and proposal of Streptomyces luteireticuli ( ex Katoh and Arai 1957)coring., sp. nov., nom. rev. Int J Syst Evol Microbiol 53, 1519–1529 [CrossRef]
HayakawaM.,
NonomuraH.1987; Humic acid-vitamin agar, a new medium for selective isolation of soil actinomycetes. J Ferment Technol 65:501–509[CrossRef]
JonesJ.1949; Fresh isolates of actinomycetes in which the presence of sporangenous aerial mycelia is a fluctuating characteristic. J Bacteriol 57:141–145
JonesA. L.,
KoernerR. J.,
NatarajanS.,
PerryJ. D.,
GoodfellowM.2008; Dietzia papillomatosis sp. nov., a novel actinomycete isolated from the skin of an immunocompetent patient with confluent and reticulated papillomatosis. Int J Syst Evol Microbiol 58:68–72[CrossRef]
LechevalierM. P.,
LechevalierH. A.1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443[CrossRef]
MayilrajS.,
SureshK.,
KroppenstedtR. M.,
SainiH. S.2006; Dietzia kunjamensis sp. nov., isolated from the Indian Himalayas. Int J Syst Evol Microbiol 56:1667–1671[CrossRef]
MinnikinD. E.,
O'DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalA.,
ParlettJ. H.1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241[CrossRef]
RaineyF. A.,
KlatteS.,
KroppenstedtR. M.,
StackebrandtE.1995; Dietzia , a new genus including Dietzia maris comb. nov., formerly Rhodococcus maris
. Int J Syst Bacteriol 45:32–36[CrossRef]
SchaalK. P.1985; Identification of clinically significant actinomycetes and related bacteria using chemical techniques. In Chemical Methods in Bacterial Systematics pp 359–381 Edited by
GoodfellowM.,
MinnikinD. E.
London: Academic Press;
StackebrandtE.,
RaineyF. A.,
Ward-RaineyN. L.1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491[CrossRef]
TakahashiK.,
NeiM.2000; Efficiencies of fast algorithms of phylogenetic inference under the criteria of maximum parsimony, minimum evolution, and maximum likelihood when a large number of sequences are used. Mol Biol Evol 17:1251–1258[CrossRef]
TamuraT.,
HatanoK.2001; Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and ‘ Actinoplanes aurantiacus ' to Cryptosporangium minutisporangium comb.nov. and Cryptosporangium aurantiacum sp. nov. Int J Syst Evol Microbiol 51:2119–2125[CrossRef]
TamuraT.,
NakagaitoY.,
NishiiT.,
HasegawaT.,
StackebrandtE.,
YokotaA.1994 A new genus of the order Actinomycetales , Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb.nov. and Couchioplanescaeruleus subsp. azureus subsp. nov. Int J Syst Bacteriol 44, 193–203 [CrossRef]
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882[CrossRef]
WilliamsS. T.,
GoodfellowM.,
AldersonG.,
WellingtonE. M. H.,
SneathP. H. A.,
SackinM. J.1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813
YassinA. F.,
HupferH.,
SchaalK. P.2006; Dietzia cinnamea sp. nov., a novel species isolated from a perianal swab of a patient with a bone marrow transplant. Int J Syst Evol Microbiol 56:641–645[CrossRef]