1887

Abstract

Four Gram-stain-positive, aerobic or facultatively anaerobic, motile, endospore-forming, rod-shaped bacteria, designated strains FeL05, FeL11, Fek19 and Fek21, were isolated from seeds of hybrid rice ( L. Jinyou 611), and their taxonomic positions were determined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that the four strains were members of the genus . They showed 95.4 % sequence similarity or less with strains of other species. The G+C content of strain FeL05 was found to be 53.3 mol%. Its predominant respiratory quinone was MK-7. The predominant cellular fatty acids were anteiso-C (61.7 %), C (10.9 %), iso-C (7.0 %), anteiso-C (6.7 %) and iso-C (5.2 %). On the basis of its phenotypic properties and phylogenetic distinctiveness, strain FeL05 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain FeL05 (=ACCC 10718 =CGMCC 1.8907 =DSM 22170).

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2010-06-01
2020-01-28
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References

  1. Beatty, P. H. & Jensen, S. E. ( 2002; ). Paenibacillus polymyxa produces fusaricidin-type antifungal antibiotics active against Leptosphaeria maculans, the causative agent of blackleg disease of canola. Can J Microbiol 48, 159–169.[CrossRef]
    [Google Scholar]
  2. Chung, Y. R., Kim, C. H., Hwang, I. & Chun, J. ( 2000; ). Paenibacillus koreensis sp. nov., a new species that produces an iturin-like antifungal compound. Int J Syst Evol Microbiol 50, 1495–1500.[CrossRef]
    [Google Scholar]
  3. Claus, D. & Berkeley, R. C. W. ( 1986; ). Genus Bacillus Cohn 1872, 174AL. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1105–1139. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  4. Collins, M. D. ( 1985; ). Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  5. Collins, M. D. & Jones, D. ( 1980; ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48, 459–470.[CrossRef]
    [Google Scholar]
  6. Eck, R. V. & Dayhoff, M. O. ( 1966; ). Atlas of Protein Sequence and Structure. Silver Springs, MD: National Biomedical Research Foundation.
  7. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  8. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  9. Helbig, J. ( 2001; ). Biological control of Botrytis cinerea Pers. ex Fr. in strawberry by Paenibacillus polymyxa (isolate 18191). J Phytopathol 149, 265–273.[CrossRef]
    [Google Scholar]
  10. Hucker, G. J. & Conn, H. J. ( 1923; ). Method of Gram staining. N Y State Agric Exp Stn Tech Bull 93, 3–37.
    [Google Scholar]
  11. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  12. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  13. Lebuhn, M., Heulin, T. & Hartmann, A. ( 1997; ). Production of auxin and other indolic and phenolic compounds by Paenibacillus polymyxa strains isolated from different proximity to plant roots. FEMS Microbiol Ecol 22, 325–334.[CrossRef]
    [Google Scholar]
  14. Lee, J.-C. & Yoon, K.-H. ( 2008; ). Paenibacillus woosongensis sp. nov., a xylanolytic bacterium isolated from forest soil. Int J Syst Evol Microbiol 58, 612–616.[CrossRef]
    [Google Scholar]
  15. MacKenzie, S. L. ( 1987; ). Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70, 151–160.
    [Google Scholar]
  16. Maes, M. & Baeyen, S. ( 2003; ). Experiences and perspectives for the use of a Paenibacillus strain as plant protectant. Commun Agric Appl Biol Sci 68, 457–462.
    [Google Scholar]
  17. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  18. Marmur, J. & Doty, P. ( 1962; ). Determination of base composition of deoxyribonucleic acid from its denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  19. Minnikin, D. E., Collins, M. D. & Goodfellow, M. ( 1979; ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47, 87–95.[CrossRef]
    [Google Scholar]
  20. Priest, F. G. ( 1977; ). Extracellular enzyme synthesis in the genus Bacillus. Bacteriol Rev 41, 711–753.
    [Google Scholar]
  21. Qiu, F. B., Huang, Y., Sun, L., Zhang, X. X., Liu, Z. H. & Song, W. ( 2007; ). Leifsonia ginsengi sp. nov., isolated from ginseng root. Int J Syst Evol Microbiol 57, 405–408.[CrossRef]
    [Google Scholar]
  22. Rivas, R., Gutiérrez, C., Abril, A., Mateos, P. F., Martínez-Molina, E., Ventosa, A. & Velázquez, E. ( 2005; ). Paenibacillus rhizosphaerae sp. nov., isolated from the rhizosphere of Cicer arietinum. Int J Syst Evol Microbiol 55, 1305–1309.[CrossRef]
    [Google Scholar]
  23. Rzhetsky, A. & Nei, M. ( 1992; ). A simple method for estimating and testing minimum evolution trees. Mol Biol Evol 9, 945–967.
    [Google Scholar]
  24. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  25. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, Technical note 101. Newark, DE: MIDI, Inc.
  26. Schleifer, K. H. ( 1985; ). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123–156.
    [Google Scholar]
  27. Slepecky, R. & Hemphill, E. ( 1992; ). The genus Bacillus – nonmedical. In The Prokaryotes, 2nd edn, pp. 1663–1696. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  28. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  29. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  30. Ten, L. N., Baek, S.-H., Im, W.-T., Lee, M., Oh, H. W. & Lee, S.-T. ( 2006; ). Paenibacillus panacisoli sp. nov., a xylanolytic bacterium isolated from soil in a ginseng field in South Korea. Int J Syst Evol Microbiol 56, 2677–2681.[CrossRef]
    [Google Scholar]
  31. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  32. Timmusk, S. & Wagner, E. G. ( 1999; ). The plant-growth-promoting rhizobacterium Paenibacillus polymyxa induces changes in Arabidopsis thaliana gene expression: a possible connection between biotic and abiotic stress responses. Mol Plant Microbe Interact 12, 951–959.[CrossRef]
    [Google Scholar]
  33. Timmusk, S., Nicander, B., Granhall, U. & Tillberg, E. ( 1999; ). Cytokinin production by Paenibacillus polymyxa. Soil Biol Biochem 31, 1847–1852.[CrossRef]
    [Google Scholar]
  34. von der Weid, I., Alviano, D. S., Santos, A. L., Soares, R. M., Alviano, C. S. & Seldin, L. ( 2003; ). Antimicrobial activity of Paenibacillus peoriae strain NRRL BD-62 against a broad spectrum of phytopathogenic bacteria and fungi. J Appl Microbiol 95, 1143–1151.[CrossRef]
    [Google Scholar]
  35. Weon-Taek, S., Kahng, G. G., Nam, S. H., Choi, S. D., Suh, H. H., Kim, S. W. & Park, Y. H. ( 1999; ). Isolation and characterization of a novel exopolysaccharide-producing Paenibacillus sp. WN9 KCTC 8951P. J Microbiol Biotechnol 9, 820–825.
    [Google Scholar]
  36. Wu, C., Lu, X., Qin, M., Wang, Y. & Ruan, J. ( 1989; ). The analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16, 176–178.
    [Google Scholar]
  37. Yoon, J. H., Oh, H. M., Yoon, B. D., Kang, K. H. & Park, Y. H. ( 2003; ). Paenibacillus kribbensis sp. nov. and Paenibacillus terrae sp. nov., bioflocculants for efficient harvesting of algal cells. Int J Syst Evol Microbiol 53, 295–301.[CrossRef]
    [Google Scholar]
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Negatively stained transmission electron micrograph of a single cell of strain FeL05 , showing peritrichous flagella and capsules around the cell. Bar, 1 µm.

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