1887

Abstract

Nine isolates from , , (Tunisia) and (Senegal) were analysed by multilocus sequence analysis (MLSA) of five housekeeping genes (, , , and ), the 16S rRNA gene and the nodulation gene . Analysis of the individual and concatenated gene sequences demonstrated that the nine new strains constituted three stable, well-supported (bootstrap and gene sequence similarity values) monophyletic clusters, A, B and C, all belonging to the branch of the genus , regardless of the phylogenetic reconstruction method used (maximum likelihood, maximum-parsimony, neighbour-joining). The three groups were further characterized by API 100 auxanographic tests, host specificity and gene sequence analysis. On the basis of these data, clusters A and C are suggested as representing two novel species within the genus , for which the names sp. nov. (type strain ORS 1407=LMG 24690=CIP 109850) and sp. nov. (type strain ORS 1400=LMG 24692=CIP 109916) are proposed. The cluster B strains were assigned to genomovar A.

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2010-03-01
2019-10-15
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Primers used for DNA amplification and sequencing. [ PDF] 62 KB

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Carbon assimilation tests. [ PDF] 141 KB

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Phylogenetic trees for five housekeeping genes ( , , , , ) and the concatenated tree of Tunisian and Senegalese strains compared with species reference strains. [ PDF] 85 KB

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vol. , part 3, pp. 664 - 674

An error has been introduced to Table 1 on page 666 of this paper. In the second line of the first column of the table, the entry for strain ORS 1444 of sp. nov. should read:

ORS 1444 (=LMG 24691)

A corrected version of the table can be seen below.



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