1887

Abstract

An endophytic actinomycete strain, designated YIM 65293, was isolated from a surface-sterilized stem sample of collected from Yunnan province, south-west China, and its taxonomic position was investigated. The chemical and morphological properties of the organism were consistent with those of the genus . Phylogenetic analysis indicated that the levels of 16S rRNA gene sequence similarity between strain YIM 65293 and other type strains of recognized members of the genus were 97.0–98.2 %. However, the DNA–DNA hybridization values, in combination with differences in phenotypic characteristics, revealed that the strain differed from recognized species of the genus . Therefore, strain YIM 65293 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 65293 (=CCTCC AA 208061=DSM 45271).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.012021-0
2009-12-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/12/3150.html?itemId=/content/journal/ijsem/10.1099/ijs.0.012021-0&mimeType=html&fmt=ahah

References

  1. Collins, M. D. & Jones, D. ( 1980; ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48, 459–470.[CrossRef]
    [Google Scholar]
  2. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  3. Coombs, J. T. & Franco, C. M. M. ( 2003; ). Isolation and identification of actinobacteria from surface-sterilized wheat roots. Appl Environ Microbiol 69, 5603–5608.[CrossRef]
    [Google Scholar]
  4. Eke, M. A., Alderson, G. & Cross, T. ( 1989; ). An actinomycete that changes wall composition during sporulation. Lett Appl Microbiol 9, 1–4.[CrossRef]
    [Google Scholar]
  5. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  7. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef]
    [Google Scholar]
  8. He, L., Li, W., Huang, Y., Wang, L. M., Liu, Z. H., Lanoot, B. J., Vancanneyt, M. & Swings, J. ( 2005; ). Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. Int J Syst Evol Microbiol 55, 1939–1944.[CrossRef]
    [Google Scholar]
  9. Itoh, T., Kudo, T., Parenti, F. & Seino, A. ( 1989; ). Amended description of the genus Kineosporia, based on chemotaxonomic and morphological studies. Int J Syst Bacteriol 39, 168–173.[CrossRef]
    [Google Scholar]
  10. Kelly, K. L. ( 1964; ). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office.
  11. Kimura, M. ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  12. Kudo, T., Matsushima, K., Itoh, T., Sasaki, J. & Suzuki, K. ( 1998; ). Description of four new species of the genus Kineosporia: Kineosporia succinea sp. nov., Kineosporia rhizophila sp. nov., Kineosporia mikuniensis sp. nov. and Kineosporia rhamnosa sp. nov., isolated from plant samples, and amended description of the genus Kineosporia. Int J Syst Bacteriol 48, 1245–1255.[CrossRef]
    [Google Scholar]
  13. Li, W. J., Xu, P., Schumann, P., Zhang, Y. Q., Pukall, R., Xu, L. H., Stackebrandt, E. & Jiang, C. L. ( 2007; ). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57, 1424–1428.[CrossRef]
    [Google Scholar]
  14. Li, J., Zhao, G. Z., Qin, S., Zhu, W. Y., Xu, L. H. & Li, W. J. ( 2009; ). Streptomyces sedi sp. nov., isolated from a surface-sterilized tissue of Sedum sp. Int J Syst Evol Microbiol 59, 1492–1496.[CrossRef]
    [Google Scholar]
  15. Locci, R. ( 1989; ). Streptomyces and related genera. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2451–2508. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  16. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  17. Minnikin, D. E., Collins, M. D. & Goodfellow, M. ( 1979; ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47, 87–95.[CrossRef]
    [Google Scholar]
  18. Pagani, H. & Parenti, F. ( 1978; ). Kineosporia, a new genus of the order Actinomycetales. Int J Syst Bacteriol 28, 401–406.[CrossRef]
    [Google Scholar]
  19. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  20. Sakiyama, Y., Thao, N. K. N., Giang, N. M., Miyadoh, S., Hop, D. V. & Andp, K. ( 2009; ). Kineosporia babensis sp. nov., isolated from plant litter in Vietnam. Int J Syst Evol Microbiol 59, 550–554.[CrossRef]
    [Google Scholar]
  21. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16
    [Google Scholar]
  22. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  23. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  24. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  25. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  26. Tamaoka, J., Katayama-Fujimura, Y. & Kuraishi, H. ( 1983; ). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54, 31–36.[CrossRef]
    [Google Scholar]
  27. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  28. Tang, S. K., Wang, Y., Lou, K., Mao, P. H., Xu, L. H., Jiang, C. L., Kim, C. J. & Li, W. J. ( 2009; ). Kocuria halotolerans sp. nov., a novel actinobacterium isolated from a saline soil in China. Int J Syst Evol Microbiol 59, 1316–1320.[CrossRef]
    [Google Scholar]
  29. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  30. Waksman, S. A. ( 1961; ). The Actinomycetes, vol. 2. Baltimore: Williams & Wilkins.
  31. Williams, S. T., Goodfellow, M. & Alderson, G. ( 1989; ). Genus Streptomyces Waksman and Henrici 1943, 339AL. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2452–2492. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.012021-0
Loading
/content/journal/ijsem/10.1099/ijs.0.012021-0
Loading

Data & Media loading...

Scanning electron micrographs of substrate mycelia and a single spore of strain YIM 65293 after growth on ISP 2 agar at 28°C for 14 days. [ PDF] 401 KB

PDF

Fatty acid contents of strain YIM 65293 and three type strains of the genus [ PDF] 35 KB

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error