1887

Abstract

A novel Gram-stain-positive, mycelium-forming actinobacterium (05-Be-005) isolated from the wall of an indoor environment was studied for its taxonomic position. The isolated strain formed a substrate mycelium that fragmented into rod-shaped cells and showed an aerial mycelium on medium M79. On the basis of 16S rRNA gene sequence similarity studies, strain 05-Be-005 was shown to belong to the genus , closely related to DSM 43194 (96.6 % similarity), YIM 90005 (95.9 %) and YIM 90001 (95.4 %). The predominant menaquinone was MK-9(H); whole-cell hydrolysates contained -diaminopimelic acid as the diagnostic diamino acid of the cell wall and arabinose and galactose as the main sugars. Mycolic acids were absent. The polar lipid profile consisted of the lipids diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylserine and an unknown phospholipid. Major fatty acids C iso, C, C 8 and C 6 supported the affiliation of strain 05-Be-005 to the genus . The results of physiological and biochemical tests allowed clear phenotypic differentiation of strain 05-Be-005 from the three known species. Strain 05-Be-005 represents a novel species, for which we propose the name sp. nov., with the type strain 05-Be-005 (=CCUG 57426 =NRRL B-24780 =CCM 7635=DSM 45305).

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2010-02-01
2019-10-22
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References

  1. Becker, B., Lechevalier, M. P. & Lechevalier, H. A. ( 1965; ). Chemical composition of cell-wall preparations from strains of various form-genera of aerobic actinomycetes. Appl Microbiol 13, 236–243.
    [Google Scholar]
  2. Chakrabarty, A. M. & Brown, J. F. ( 1978; ). Microbial genetic engineering by natural plasmid transfer. In Genetic Engineering, pp. 185–193. Edited by A. M. Chakrabarty. West Palm Beach, FL: CRC Press.
  3. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  4. Collins, M. D., Goodfellow, M. & Minnikin, D. E. ( 1980; ). Fatty acid, isoprenoid quinone and polar lipid composition in the classification of Curtobacterium and related taxa. J Gen Microbiol 118, 29–37.
    [Google Scholar]
  5. Gauze, G. F., Preobrazhenskaya, T. P., Sveshnikova, M. A., Terekova, L. P. & Maksimova, T. S. ( 1983; ). Opredelitel'Aktinomycetov. Rody Streptomyces, Streptoverticillium, Chainia. Moscow: Izd. Nauka (in Russian).
  6. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  7. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  8. Kämpfer, P. & Kroppenstedt, R. M. ( 2004; ). Pseudonocardia benzenivorans sp. nov. Int J Syst Evol Microbiol 54, 749–751.[CrossRef]
    [Google Scholar]
  9. Kämpfer, P., Steiof, M. & Dott, W. ( 1991; ). Microbiological characterisation of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21, 227–251.[CrossRef]
    [Google Scholar]
  10. Kim, S. B. & Goodfellow, M. ( 1999; ). Reclassification of Amycolatopsis rugosa Lechevalier et al. 1986 as Prauserella rugosa gen. nov., comb. nov. Int J Syst Bacteriol 49, 507–512.[CrossRef]
    [Google Scholar]
  11. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  12. Lechevalier, M. P. & Lechevalier, H. A. ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef]
    [Google Scholar]
  13. Lechevalier, M. P., De Bièvre, C. & Lechevalier, H. A. ( 1977; ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef]
    [Google Scholar]
  14. Li, W.-J., Xu, P., Tang, S.-K., Xu, L.-H., Kroppenstedt, R. M., Stackebrandt, E. & Jiang, C.-L. ( 2003; ). Prauserella halophila sp. nov. and Prauserella alba sp. nov., moderately halophilic actinomycetes from saline soil. Int J Syst Evol Microbiol 53, 1545–1549.[CrossRef]
    [Google Scholar]
  15. Minnikin, D. E., Alshamaony, L. & Goodfellow, M. ( 1975; ). Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88, 200–204.[CrossRef]
    [Google Scholar]
  16. Minnikin, D. E., Collins, M. D. & Goodfellow, M. ( 1979; ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47, 87–95.[CrossRef]
    [Google Scholar]
  17. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  18. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
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