1887

Abstract

A novel strain, designated TP2-8, was isolated from fermented fish () in Thailand. It stained Gram-positive and the cells were aerobic, endospore-forming rods. The strain grew at pH 6–8 (optimum pH 7), 15–55 °C (optimum 37 °C) and 1–22 % (w/v) NaCl (optimum 5–10 %). It contained -diaminopimelic in the cell-wall peptidoglycan. MK-7 and cellular fatty acids anteiso-C, iso-C and anteiso-C were major components. Polar lipids diphosphatidylglycerol and phosphatidylglycerol and unidentified lipids were detected. The DNA G+C content was 37.6 mol%. Comparison of the 16S rRNA gene sequence of strain TP2-8 with those of other members of the family indicated that it was a member of the genus (94.9–99.2 % sequence similarity) and was closely related to YIM 91119 (99.2 % similarity), BH312 (98.6 %), XH-63 (97.7 %), ‘’ YIM C229 (97.7 %) and T-16X (97.2 %). Strain TP2-8 showed low DNA–DNA relatedness (≤49 %) to YIM 91119, DSM 19029, CCM 7326, ‘’ DSM 17858 and JCM 21714. On the basis of the physiological and biochemical characteristics and molecular data presented, strain TP2-8 is proposed to represent a novel species, sp. nov. (type strain TP2-8 =JCM 15569 =PCU 304 =TISTR 1881).

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2010-04-01
2019-12-15
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vol. , part 4, pp. 944 - 948

Cellular fatty acid compositions of strain TP2-8 and type strains of species.

DNA–DNA relatedness between strain TP2-8 and type strains of species.

Phylogenetic trees generated by the maximum-parsimony (Fig. S1) and maximum-likelihood (Fig. S2) methods showing relationships between strain TP2-8 , species and related taxa based on 16S rRNA gene sequences.

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