1887

Abstract

The taxonomic position of an actinomycete strain YIM 63135, which was isolated from the surface-sterilized tissue of L. collected from Yunnan province, south-west China, was determined by using a polyphasic approach. Morphological and chemical characteristics of the novel strain were consistent with those of the genus . It developed a pinkish aerial mycelium and pinkish-brown substrate mycelium on oatmeal agar. The cell wall of the strain contained -diaminopimelic acid. The menaquinones comprised MK-9(H) (62.8 %), MK-9(H) (31.4 %) and MK-9(H) (5.9 %). The phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, an unknown glucosamine-containing phospholipid (GluNu), phosphatidylinositol mannosides and four unknown ninhydrin-negative phospholipids. The major fatty acids were iso-C (30.0 %), anteiso-C (27.3 %) and anteiso-C (17.0 %). The DNA GC content of strain YIM 63135 was 72.6 mol%. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain YIM 63135 is a member of the genus and exhibited 99.9 % gene sequence similarity to NBRC 12555, while low sequence similarity values (<97.0 %) distinguished strain YIM 63135 from all other species. DNA–DNA hybridization studies suggested that strain YIM 63135 represents a different genomic species. On the basis of phenotypic and phylogenetic characteristics, strain YIM 63135 was considered to represent a novel species of the genus , for which the name sp. nov. is proposed, with YIM 63135 (=CCTCC AA 208059 =DSM 41953) as the type strain.

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2010-01-01
2021-10-27
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