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Abstract

A taxonomic study was carried out on strain LMC2up-L3, which was isolated from a polycyclic aromatic hydrocarbon-degrading consortium enriched with a sediment sample collected from a hydrothermal field of the south-west Pacific Ocean. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LMC2up-L3 belonged to the genus , with the highest sequence similarity of 98.4 % to P24; similarity to other strains was below 93.1 %. DNA–DNA hybridization between strain LMC2up-L3 and P24 was 31 %. rep-PCR fingerprints also differentiated strain LMC2up-L3 from P24. The G+C content of the chromosomal DNA was 67.7 mol%. The principal fatty acids were C (17.8 %), C (21.2 %), C 7 (23.6 %), C (4.1 %), C 2-OH (4.5 %) and C cyclo 8 (17.4 %). The combined genotypic and phenotypic data show that strain LMC2up-L3 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain LMC2up-L3 (=CCTCC AB 209059 =LMG 24859 =MCCC 1A02656). An emended description of the genus is also provided.

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2010-01-01
2024-03-28
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References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J., Struhl K. A. (editors) 1995 Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology , 3rd edn. New York: Wiley;
    [Google Scholar]
  2. Biebl H., Allgaier M., Tindall B. J., Koblizek M., Lünsdorf H., Pukall R., Wagner-Döbler I. 2005; Dinoroseobacter shibae gen. nov., sp. nov. a new aerobic phototrophic bacterium isolated from dinoflagellates. Int J Syst Evol Microbiol 55:1089–1096 [CrossRef]
    [Google Scholar]
  3. Dong X.-Z., Cai M.-Y. 2001 Determinative Manual for Routine Bacteriology Beijing: Scientific Press (English translation;
    [Google Scholar]
  4. Ivanova E. P., Kiprianova E. A., Mikhailov V. V., Levanova G. F., Garagulya A. D., Gorshkova N. M., Vysotskii M. V., Nicolau D. V., Yumoto N. other authors 1998; Phenotypic diversity of Pseudoalteromonas citrea from different marine habitats and emendation of the description. Int J Syst Bacteriol 48:247–256 [CrossRef]
    [Google Scholar]
  5. Lai Q., Yuan J., Wu C., Shao Z. 2009; Oceanibaculum indicum gen. nov., sp. nov. isolated from deep seawater of the Indian Ocean. Int J Syst Evol Microbiol 59:1733–1737 [CrossRef]
    [Google Scholar]
  6. Liu C., Shao Z. 2005; Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55:1181–1186 [CrossRef]
    [Google Scholar]
  7. Mesbah M., Whitman W. B. 1989; Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J Chromatogr 479:297–306 [CrossRef]
    [Google Scholar]
  8. Rzhetsky A., Nei M. 1992; A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 9:945–967
    [Google Scholar]
  9. Rzhetsky A., Nei M. 1993; Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 10:1073–1095
    [Google Scholar]
  10. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  11. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  12. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids . MIDI Technical Note 101: Newark, DE: MIDI;
    [Google Scholar]
  13. Shieh W. Y., Chen Y.-W., Chaw S.-M., Chiu H.-H. 2003; Vibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water. Int J Syst Evol Microbiol 53:479–484 [CrossRef]
    [Google Scholar]
  14. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  15. Versalovic J., Koeuth T., Lupski J. R. 1991; Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 19:6823–6831 [CrossRef]
    [Google Scholar]
  16. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
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