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Abstract

A taxonomic study was carried out on strain LMC2up-L3, which was isolated from a polycyclic aromatic hydrocarbon-degrading consortium enriched with a sediment sample collected from a hydrothermal field of the south-west Pacific Ocean. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LMC2up-L3 belonged to the genus , with the highest sequence similarity of 98.4 % to P24; similarity to other strains was below 93.1 %. DNA–DNA hybridization between strain LMC2up-L3 and P24 was 31 %. rep-PCR fingerprints also differentiated strain LMC2up-L3 from P24. The G+C content of the chromosomal DNA was 67.7 mol%. The principal fatty acids were C (17.8 %), C (21.2 %), C 7 (23.6 %), C (4.1 %), C 2-OH (4.5 %) and C cyclo 8 (17.4 %). The combined genotypic and phenotypic data show that strain LMC2up-L3 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain LMC2up-L3 (=CCTCC AB 209059 =LMG 24859 =MCCC 1A02656). An emended description of the genus is also provided.

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2010-01-01
2020-01-19
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rep-PCR comparison of strain LMC2up-L3 (lane 1) and P24 (lane 2) using the BOX-A1R primer. Lane M1, 500 bp DNA ladder; M2, 100 bp DNA ladder.

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Transmission electron micrographs of negatively stained cells of strain LMC2up-L3 grown on 216L agar for 24 h. Bars, 1 µm.

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Transmission electron micrographs of negatively stained cells of strain LMC2up-L3 grown on 216L agar for 24 h. Bars, 1 µm.

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Cellular fatty acid contents of strain LMC2up-L3 and P24 . [PDF](53 KB)

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