1887

Abstract

An ivory-coloured bacterium, designated strain 5YN7-3, was isolated from a wetland, Yongneup, Korea. Cells of the strain were aerobic, Gram-stain-negative, non-motile and short rods. 16S rRNA gene sequence analysis demonstrated that strain 5YN7-3 belongs to the order of the class and is closely related to 5YN9-8 (97.8 %), Ko04 (97.6 %) and Chj404 (97.4 %). Strain 5YN7-3 showed DNA–DNA hybridization values of 28, 22 and 35 % with Ko04, 5YN9-8 and Chj404, respectively. The major fatty acids were C 7 (51.2 %), C cyclo 8 (25.0 %), C (12.9 %) and C (10.8 %) (>10 % of total fatty acids). Ubiquinone-10 was the major isoprenoid quinone and the DNA G+C content was 66.5 mol%. The phenotypic characteristics in combination with 16S rRNA gene sequence analysis and DNA–DNA hybridization data clearly define strain 5YN7-3 as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 5YN7-3 (=KACC 12910 =DSM 21341).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.011924-0
2010-04-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/4/949.html?itemId=/content/journal/ijsem/10.1099/ijs.0.011924-0&mimeType=html&fmt=ahah

References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A. Struhl K. (editors) 1987 Current Protocols in Molecular Biology New York: Wiley;
    [Google Scholar]
  2. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  3. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  4. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  5. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [CrossRef]
    [Google Scholar]
  6. Im W. T., Yokota A., Kim M. K., Lee S. T. 2004; Kaistia adipata gen. nov., sp. nov., a novel α -proteobacterium. J Gen Appl Microbiol 50:249–254 [CrossRef]
    [Google Scholar]
  7. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press;
    [Google Scholar]
  8. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  9. Kwon S. W., Kim J. S., Park I. C., Yoon S. H., Park D. H., Lim C. K., Go S. J. 2003; Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. Int J Syst Evol Microbiol 53:21–27 [CrossRef]
    [Google Scholar]
  10. Kwon S.-W., Kim B.-Y., Kim W.-G., Yoo K.-H., Yoo S.-H., Son J.-A., Weon H.-W. 2008; Paludibacterium yongneupense gen. nov., sp. nov., isolated from a wetland, Yongneup, in Korea. Int J Syst Evol Microbiol 58:190–194 [CrossRef]
    [Google Scholar]
  11. Lee H.-W., Yu H.-S., Liu Q.-M., Jung H.-M., An D.-S., Im W.-T., Jin F.-X., Lee S.-T. 2007; Kaistia granuli sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor. Int J Syst Evol Microbiol 57:2280–2283 [CrossRef]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid-chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  13. Reasoner D. J., Geldreich E. E. 1985; A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 49:1–7
    [Google Scholar]
  14. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  15. Seldin L., Dubnau D. 1985; Deoxyribonucleic acid homology among Bacillus polymyxa , Bacillus macerans , Bacillus azotofixans , and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35:151–154 [CrossRef]
    [Google Scholar]
  16. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology , pp 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  17. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  18. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  19. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
    [Google Scholar]
  20. Weon H.-Y., Kim B.-Y., Yoo S.-H., Kwon S.-W., Go S.-J., Stackebrandt E. 2008a; Uliginosibacterium gangwonense gen. nov., sp. nov., isolated from a wetland, Yongneup, in Korea. Int J Syst Evol Microbiol 58:131–135 [CrossRef]
    [Google Scholar]
  21. Weon H.-Y., Lee C.-M., Hong S.-B., Kim B.-Y., Yoo S.-H., Kwon S.-W., Go S.-J. 2008b; Kaistia soli sp. nov., isolated from a wetland in Korea. Int J Syst Evol Microbiol 58:1522–1524 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.011924-0
Loading
/content/journal/ijsem/10.1099/ijs.0.011924-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error