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Volume 59, Issue 12

gen. nov., sp. nov., a glutamate-degrading, Gram-positive anaerobic coccus isolated from a methanogenic reactor treating cattle waste Free

Abstract

A strictly anaerobic, mesophilic and aminolytic strain (WN036) was isolated from a methanogenic reactor treating waste from cattle farms. Cells were Gram-positive cocci, often occurred in pairs and were non-motile. Although spore formation was not confirmed by microscopic observation of cells, the strain produced thermotolerant cells. The optimum temperature for growth was 35–37 °C and the optimum pH was 6.7. Oxidase, catalase and nitrate-reducing activities were negative. The novel strain did not ferment carbohydrates and grew in PY medium without additional substrates. The strain utilized -glutamate, -glutamine, -histidine and -arginine as growth substrates. Major fermentation products were acetate and butyrate with a small amount of propionate. The genomic DNA G+C content was 32.5 mol%. The major cellular fatty acids were C 8, C 7 DMA and C. The diagnostic diamino acid of the cell-wall peptidoglycan was lysine. Glutamic acid, glycine, alanine and aspartic acid were also detected in the cell-wall peptidoglycan. On the basis of 16S rRNA gene sequences, the most closely related species to strain WN036 were ATCC 14965 (89.8 %) and ATCC 29427 (89.6 %). Based on the differences in the phenotypic and phylogenetic characteristics of strain WN036 compared with those of closely related species, a novel genus and species, gen. nov., sp. nov., is proposed. The type strain is WN036 (=JCM 15094=DSM 21120).

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Keyword(s): CFA, cellular fatty acid , DMA, dimethylacetal , GPAC, Gram-positive, anaerobic cocci and VFA, volatile fatty acid
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2009-12-01
2023-09-23
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Anaerosphaera aminiphila gen. nov., sp. nov., a glutamate-degrading, Gram-positive anaerobic coccus isolated from a methanogenic reactor treating cattle waste
Int J Syst Evol Microbiol 59, 3161 (2009); https://doi.org/10.1099/ijs.0.011858-0
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