1887

Abstract

Gram-negative, coccoid, non-motile bacteria that are catalase-, urease- and indole-negative, facultatively anaerobic and oxidase-positive were isolated from the bovine rumen using an improved selective medium for members of the . All strains produced significant amounts of succinic acid under anaerobic conditions with glucose as substrate. Phenotypic characterization and multilocus sequence analysis (MLSA) using 16S rRNA, , and genes were performed on seven independent isolates. All four genes showed high sequence similarity to their counterparts in the genome sequence of the patent strain MBEL55E, but less than 95 % 16S rRNA gene sequence similarity to any other species of the . Genetically these strains form a very homogeneous group in individual as well as combined phylogenetic trees, clearly separated from other genera of the family from which they can also be separated based on phenotypic markers. Genome relatedness as deduced from the gene showed high interspecies similarities, but again low similarity to any of the established genera of the family. No toxicity towards bovine, human or fish cells was observed and no RTX toxin genes were detected in members of the new taxon. Based on phylogenetic clustering in the MLSA analysis, the low genetic similarity to other genera and the phenotypic distinction, we suggest to classify these bovine rumen isolates as gen. nov., sp. nov. The type strain is JF4016 (=DSM 22022 =CCUG 57335).

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2010-01-01
2019-10-17
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vol. , part 1, pp. 44 - 50

[ PDF], 442 KB, containing:

Phylogenetic tree based on 16S rRNA gene sequences.

Phylogenetic tree based on rpoB gene sequences.

Phylogenetic tree based on infB gene sequences.

Phylogenetic tree based on recN gene sequences.

gen. nov., sp. nov. isolates from bovine rumen



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