@article{mbs:/content/journal/ijsem/10.1099/ijs.0.011775-0, author = "Reddy, G. S. N. and Pradhan, Suman and Manorama, Ruth and Shivaji, S.", title = "Cryobacterium roopkundense sp. nov., a psychrophilic bacterium isolated from glacial soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2010", volume = "60", number = "4", pages = "866-870", doi = "https://doi.org/10.1099/ijs.0.011775-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.011775-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Strain RuGl7T was isolated from a soil sample collected at the periphery of the glacial Lake Roopkund in the Himalayan mountain range, India. Cells of RuGl7T were Gram-positive, aerobic, rod-shaped, motile and grew optimally between 15 and 18 °C. Cells of RuGl7T contained 2,4-diaminobutyric acid in the cell-wall peptidoglycan and the major menaquinones were MK-10, MK-11 and MK-12. The polar lipids present were diphosphatidylglycerol and phosphatidylglycerol and an unknown lipid and the major fatty acid was anteiso-C15 : 0. Based on the above characteristics, strain RuGl7T was assigned to the genus Cryobacterium. Strain RuGl7T shared a 16S rRNA gene sequence similarity of 97.0 and 99.0 % with Cryobacterium psychrotolerans JCM 13925T and Cryobacterium psychrophilum JCM 1463T, respectively. However, DNA–DNA relatedness values between strain RuGl7T and C. psychrotolerans and C. psychrophilum were 28 and 23 %, respectively. Furthermore, strain RuGl7T exhibited several phenotypic and genotypic differences when compared with C. psychrotolerans, C. psychrophilum and Cryobacterium mesophilum. Based on these differentiating characteristics, strain RuGl7T was identified as a novel species of the genus Cryobacterium for which the name Cryobacterium roopkundense sp. nov. is proposed. The type strain is RuGl7T (=DSM 21065T=JCM 15131T).", }