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Abstract

A thermophilic strain, designated YIM 10002, was isolated from a soil sample of Big Empty Volcano in Tengchong county, Yunnan province, south-west China, and a polyphasic approach was used to investigate its taxonomic position. Strain YIM 10002 formed endospores on both aerial and substrate mycelia. Whole-cell hydrolysates contained -diaminopimelic acid, ribose, xylose and glucose. The major fatty acids were iso-C and anteiso-C. The predominant menaquinone was MK-9. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides, together with some unknown phospholipids. The GC content of its genomic DNA was 48.6 mol%. All of these chemotaxonomic data together with morphological characters consistently assigned strain YIM 10002 to the genus . 16S rRNA gene sequence analysis showed that strain YIM 10002 was most closely related to KCTC 9790 and KCTC 3666 (99.9 and 98.0 % 16S rRNA gene sequence similarity, respectively). However, strain YIM 10002 showed relatively low DNA–DNA relatedness (34.0 and 39.0 %, respectively) with the above strains. Therefore, strain YIM 10002 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 10002 (=DSM 45262 =CCTCC AA 208050).

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2010-09-01
2019-10-20
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Scanning electron micrograph of growth of strain YIM 10002 showing endospores on aerial mycelium after growth on ISP 3 at 55 °C for 10 days. Bar, 2 µm.

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Two-dimensional thin-layer chromatograms showing the polar lipids of strain YIM 10002 (a), DSM 43356 (b) and DSM 44608 (c). DPG, Diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PIM, phosphatidylinositol mannosides; PME, phosphatidylmonomethylethanolamine; PL, unknown phospholipid.

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Cellular fatty acid profiles of strain YIM 10002 and its closest phylogenetic neighbours. [PDF](36 KB)

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