1887

Abstract

Two strictly anaerobic, proteolytic bacterial strains, designated strain D3RC-2 and D3RC-3r, were isolated from a cellulose-degrading mixed culture enriched from yak rumen content. The strains were Gram-stain negative and non-spore-forming with cell sizes of 0.5–0.8×0.6–2.0 μm. The temperature range for growth was 24–46 °C (optimum 38–39 °C) and the pH range was between 5.6 and 8.7 (optimum 7.0–7.3). Both strains used soya peptone, tryptone, -phenylalanine, -leucine, -methionine, -serine, -valine, -threonine and -histidine as carbon and nitrogen sources, but did not use any of the saccharides tested. The major fermentation products from PY medium were acetate, propionate and iso-butyrate. The DNA G+C contents of strains D3RC-2 and D3RC-3r were 41.0±0.1 mol% and 41.3±0.1 mol% (HPLC), respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the two strains represented a new phyletic sublineage within the family , with <93.8 % 16S rRNA gene sequence similarity to recognized species. On the basis of the phenotypic, genotypic and physiological evidence, strains D3RC-2 and D3RC-3r are proposed as representing a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is D3RC-2 (=AS 1.5057=JCM 14817).

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2010-09-01
2020-09-22
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References

  1. Alain K., Pignet P., Zbinden M., Quillevere M., Duchiron F., Donval J. P., Lesongeur F., Raguenes G., Crassous P. other authors 2002; Caminicella sporogenes gen. nov., sp. nov., a novel thermophilic spore-forming bacterium isolated from an East-Pacific Rise hydrothermal vent. Int J Syst Evol Microbiol 52:1621–1628 [CrossRef]
    [Google Scholar]
  2. An D., Dong X., Dong Z. 2005; Prokaryote diversity in the rumen of yak ( Bos grunniens ) and Jinnan cattle ( Bos taurus ) estimated by 16S rRNA homology analyses. Anaerobe 11:207–215 [CrossRef]
    [Google Scholar]
  3. Bryant M. P., Burkey L. A. 1953; Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen. J Dairy Sci 36:205–217 [CrossRef]
    [Google Scholar]
  4. Canale-Parole E. 1986; Genus Sarcina Goodsir 1842. In Bergey's Manual of Systematic Bacteriology vol 2 pp 1100–1103 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams and Wilkins;
    [Google Scholar]
  5. Cao X., Liu X., Dong X. 2003; Alkaliphilus crotonatoxidans sp. nov., a strictly anaerobic, crotonate-dismutating bacterium isolated from a methanogenic environment. Int J Syst Evol Microbiol 53:971–975 [CrossRef]
    [Google Scholar]
  6. Cayol J.-L., Ducerf S., Patel B. K. C., Garcia J.-L., Thomas P., Ollivier B. 2000; Thermohalobacter berrensis gen. nov., sp. nov. a thermophilic, strictly halophilic bacterium from a solar saltern. Int J Syst Evol Microbiol 50:559–564 [CrossRef]
    [Google Scholar]
  7. Chen S., Dong X. 2004; Acetanaerobacterium elongatum gen. nov., sp. nov., from paper mill waste water. Int J Syst Evol Microbiol 54:2257–2262 [CrossRef]
    [Google Scholar]
  8. Christostomos S., Patel B. K. C., Dwivedi P. P., Denman S. E. 1996; Caloramator indicus sp. nov., a new thermophilic anaerobic bacterium isolated from the deep-seated nonvolcanically heated waters of an Indian artesian aquifer. Int J Syst Bacteriol 46:497–501 [CrossRef]
    [Google Scholar]
  9. Duda V. I., Lebedinsky A. V., Mushegjan M. S., Mitjushina L. L. 1987; A new anaerobic bacterium, forming up to five endospores per cell – Anaerobacter polyendosporus gen. et spec. nov. Arch Microbiol 148:121–127 [CrossRef]
    [Google Scholar]
  10. Engle M., Li Y., Rainey F., DeBlois S., Mai V., Reichert A., Mayer F., Messner P., Wiegel J. 1996; Thermobrachium celere gen. nov., sp. nov., a rapidly growing thermophilic, alkalitolerant, and proteolytic obligate anaerobe. Int J Syst Bacteriol 46:1025–1033 [CrossRef]
    [Google Scholar]
  11. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  12. Garnova E. S., Zhilina T. N., Tourova T. P., Lysenko A. M. 2003; Anoxynatronum sibiricum gen. nov., sp. nov. alkaliphilic saccharolytic anaerobe from cellulolytic community of Nizhnee Beloe (Transbaikal region). Extremophiles 7:213–220
    [Google Scholar]
  13. Hippe H., Andreesen J. R., Gottschalk G. 1992; The genus Clostridium – nonmedical. In The Prokaryotes, 2nd edn. vol 2 pp 1800–1866 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
    [Google Scholar]
  14. Holdeman L. V., Cato E. P., Moore M. E. C. 1977 Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute & State University;
    [Google Scholar]
  15. Hungate R. E. 1966 The Rumen and its Microbes New York: Academic Press;
    [Google Scholar]
  16. Hungate R. E. 1969; A roll tube method for cultivation of strict anaerobes. Methods Microbiol 3B:117–132
    [Google Scholar]
  17. Johnson M. J., Thatcher E., Cox M. E. 1995; Techniques for controlling variability in gram staining of obligate anaerobes. J Clin Microbiol 33:755–758
    [Google Scholar]
  18. Kevbrin V. V., Zhilina T. N., Rainey F. A., Zavarzin G. A. 1998; Tindallia magadii gen. nov., sp. nov.: an alkaliphilic anaerobic ammonifier from soda lake deposits. Curr Microbiol 37:94–100 [CrossRef]
    [Google Scholar]
  19. Krumholz L. R., Bryant M. P. 1985; Clostridium pfennigii sp. nov. uses methoxyl groups of monobenzenoids and produces butyrate. Int J Syst Bacteriol 35:454–456 [CrossRef]
    [Google Scholar]
  20. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; mega2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  21. Lee Y.-J., Wagner I. D., Brice M. E., Kevbrin V. V., Mills G. L., Romanek C. S., Wiegel J. 2005 Thermosediminibacter oceani gen. nov., sp. nov. and Thermosediminibacter litoriperuensis sp. nov., new anaerobic thermophilic bacteria isolated from Peru Margin. Extremophiles 9375–383 [CrossRef]
  22. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  23. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  24. Miller L. T. 1982; Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxyl acids. J Clin Microbiol 16:584–586
    [Google Scholar]
  25. Patel B. K. C., Monk C., Littleworth H., Morgan H. W., Daniel R. M. 1987; Clostridium fervidus sp. nov., a new chemoorganotrophic acetogenic thermophile. Int J Syst Bacteriol 37:123–126 [CrossRef]
    [Google Scholar]
  26. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  27. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology. pp 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  28. Takai K., Moser D. P., Onstott T. C., Spoelstra N., Pfiffner S. M., Dohnalkova A., Fredrickson J. K. 2001; Alkaliphilus transvaalensis gen. nov., sp. nov. an extremely alkaliphilic bacterium isolated from a deep South African gold mine. Int J Syst Evol Microbiol 51:1245–1256
    [Google Scholar]
  29. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  30. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
    [Google Scholar]
  31. Wery N., Moricet J.-M., Cueff V., Jean J., Pignet P., Lesongeur F., Cambon-Bonavita M.-A., Barbier G. 2001; Caloranaerobacter azorensis gen. nov., sp. nov. an anaerobic thermophilic bacterium isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 51:1789–1796 [CrossRef]
    [Google Scholar]
  32. Zhilina T. N., Detkova E. N., Rainey F. A., Osipov G. A., Lysenko A. M., Kostrikina N. A., Zavarzin G. A. 1998; Natronoincola histidinovorans gen. , nov., sp. nov. a new alkaliphilic acetogenic anaerobe. Curr Microbiol 37177–185 [CrossRef]
    [Google Scholar]
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