1887

Abstract

Two strictly anaerobic, proteolytic bacterial strains, designated strain D3RC-2 and D3RC-3r, were isolated from a cellulose-degrading mixed culture enriched from yak rumen content. The strains were Gram-stain negative and non-spore-forming with cell sizes of 0.5–0.8×0.6–2.0 μm. The temperature range for growth was 24–46 °C (optimum 38–39 °C) and the pH range was between 5.6 and 8.7 (optimum 7.0–7.3). Both strains used soya peptone, tryptone, -phenylalanine, -leucine, -methionine, -serine, -valine, -threonine and -histidine as carbon and nitrogen sources, but did not use any of the saccharides tested. The major fermentation products from PY medium were acetate, propionate and iso-butyrate. The DNA G+C contents of strains D3RC-2 and D3RC-3r were 41.0±0.1 mol% and 41.3±0.1 mol% (HPLC), respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the two strains represented a new phyletic sublineage within the family , with <93.8 % 16S rRNA gene sequence similarity to recognized species. On the basis of the phenotypic, genotypic and physiological evidence, strains D3RC-2 and D3RC-3r are proposed as representing a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is D3RC-2 (=AS 1.5057=JCM 14817).

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2010-09-01
2019-12-13
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