sp. nov., a proteolytic bacterium isolated from soil, and reclassification of into the genus as comb. nov. Free

Abstract

A Gram-negative, motile and rod-shaped bacterial strain, BIO-TAS2-2, of the class , was isolated from a soil in Korea and studied using a polyphasic taxonomic approach. Strain BIO-TAS2-2 grew optimally at pH 7.5–8.5 and 30 °C and in the presence of 0–1.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain BIO-TAS2-2 fell within the clade comprising species of the genus , forming a coherent cluster with KSL-145 and LMG 2089. It exhibited 16S rRNA gene sequence similarity values of 96.0–98.7 % to members of the genus and IAM 13153. Strain BIO-TAS2-2 contained Q-10 as the predominant ubiquinone and cyclo-C 7 and C as the major fatty acids. The DNA G+C content was 67.0 mol%. Strain BIO-TAS2-2 exhibited DNA–DNA relatedness levels of 12–19 % with the type strains of phylogenetically related species and . . The novel strain could be differentiated from species and . by differences in phenotypic characteristics. On the basis of phenotypic, phylogenetic and genetic data, strain BIO-TAS2-2 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BIO-TAS2-2 (=KCTC 22631=CCUG 57609). In this study, it is also proposed that be transferred to the genus as comb. nov. (type strain TK0051=ATCC 4278=DSM 7126=JCM 20846=LMG 17157).

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2009-12-01
2024-03-28
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