Brevundimonas naejangsanensis sp. nov., a proteolytic bacterium isolated from soil, and reclassification of Mycoplana bullata into the genus Brevundimonas as Brevundimonas bullata comb. nov.
A Gram-negative, motile and rod-shaped bacterial strain, BIO-TAS2-2T, of the class Alphaproteobacteria, was isolated from a soil in Korea and studied using a polyphasic taxonomic approach. Strain BIO-TAS2-2T grew optimally at pH 7.5–8.5 and 30 °C and in the presence of 0–1.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain BIO-TAS2-2T fell within the clade comprising species of the genus Brevundimonas, forming a coherent cluster with Brevundimonasterrae KSL-145T and Brevundimonasdiminuta LMG 2089T. It exhibited 16S rRNA gene sequence similarity values of 96.0–98.7 % to members of the genus Brevundimonas and Mycoplana bullata IAM 13153T. Strain BIO-TAS2-2T contained Q-10 as the predominant ubiquinone and cyclo-C18 : 1ω7c and C16 : 0 as the major fatty acids. The DNA G+C content was 67.0 mol%. Strain BIO-TAS2-2T exhibited DNA–DNA relatedness levels of 12–19 % with the type strains of phylogenetically related Brevundimonas species and M. bullata. The novel strain could be differentiated from Brevundimonas species and M. bullata by differences in phenotypic characteristics. On the basis of phenotypic, phylogenetic and genetic data, strain BIO-TAS2-2T is considered to represent a novel species of the genus Brevundimonas, for which the name Brevundimonas naejangsanensis sp. nov. is proposed. The type strain is BIO-TAS2-2T (=KCTC 22631T=CCUG 57609T). In this study, it is also proposed that Mycoplana bullata be transferred to the genus Brevundimonas as Brevundimonas bullata comb. nov. (type strain TK0051T=ATCC 4278T=DSM 7126T=JCM 20846T=LMG 17157T).
Abraham, W.-R., Strömpl, C., Meyer, H., Lindholst, S., Moore, E. R. B., Christ, R., Vancanneyt, M., Tindall, B. J., Bennasar, A. & other authors(1999). Phylogeny and polyphasic taxonomy of Caulobacter species. Proposal of Maricaulis gen. nov. with Maricaulis maris (Poindexter) comb. nov. as the type species, and emended description of the genera Brevundimonas and Caulobacter. Int J Syst Bacteriol49, 1053–1073.[CrossRef][Google Scholar]
Cowan, S. T. & Steel, K. J.(1965).Manual for the identification of medical bacteria. London: Cambridge University Press.
Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol39, 224–229.[CrossRef][Google Scholar]
Fritz, I., Strömpl, C., Nikitin, D. I., Lysenko, A. M. & Abraham, W.-R.(2005).Brevundimonas mediterranea sp. nov., a non-stalked species from the Mediterranean Sea. Int J Syst Evol Microbiol55, 479–486.[CrossRef][Google Scholar]
Gray, P. H. H. & Thornton, H. G.(1928). Soil bacteria that decompose certain aromatic compounds. Zentralbl Bakteriol Parasitenkd Infektionskr Hyg Abt II73, 74–96.
[Google Scholar]
Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol19, 161–207.
[Google Scholar]
Lányí, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol19, 1–67.
[Google Scholar]
Li, Y., Kawamura, Y., Fujiwara, N., Naka, T., Liu, H., Huang, X., Kobayashi, K. & Ezaki, T.(2004).Sphingomonas yabuuchiae sp. nov. and Brevundimonas nasdae sp. nov., isolated from the Russian space laboratory Mir. Int J Syst Evol Microbiol54, 819–825.[CrossRef][Google Scholar]
Palleroni, N. J.(1984). Genus Pseudomonas Migula 1894. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 141–199. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
Poindexter, J. S.(1964). Biological properties and classification of the Caulobacter group. Bacteriol Rev28, 231–295.
[Google Scholar]
Poindexter, J. S.(1989). Genus Caulobacter Henrici and Johnson 1935. In Bergey's Manual of Systematic Bacteriology, vol. 3, pp. 1924–1939. Edited by J. T. Staley, M. P. Bryant, N. Pfennig & J. G. Holt. Baltimore: Williams & Wilkins.
Ryu, S. H., Park, M., Lee, J. R., Yun, P.-Y. & Jeon, C. O.(2007).Brevundimonas aveniformis sp. nov., a stalked species isolated from activated sludge. Int J Syst Evol Microbiol57, 1561–1565.[CrossRef][Google Scholar]
Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
Segers, P., Vancanneyt, M., Pot, B., Torck, U., Hoste, B., Dewettinck, D., Falsen, E., Kersters, K. & De Vos, P.(1994). Classification of Pseudomonas diminuta Leifson and Hugh 1954 and Pseudomonas vesicularis Büsing, Döll, and Freytag 1953 in Brevundimonas gen. nov. as Brevundimonas diminuta comb. nov. and Brevundimonas vesicularis comb. nov., respectively. Int J Syst Bacteriol44, 499–510.[CrossRef][Google Scholar]
Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett25, 125–128.[CrossRef][Google Scholar]
Urakami, T., Oyanagi, H., Araki, H., Suzuki, K.-I. & Komagata, K.(1990). Recharacterization and emended description of the genus Mycoplana and description of two new species, Mycoplana ramosa and Mycoplana segnis. Int J Syst Bacteriol40, 434–442.[CrossRef][Google Scholar]
Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol37, 463–464.[CrossRef][Google Scholar]
Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H.(1996). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol46, 502–505.[CrossRef][Google Scholar]
Yoon, J.-H., Lee, S. T. & Park, Y.-H.(1998). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol48, 187–194.[CrossRef][Google Scholar]
Yoon, J.-H., Kang, K. H. & Park, Y.-H.(2003).Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol53, 449–454.[CrossRef][Google Scholar]
Yoon, J.-H., Kang, S.-J., Oh, H. W., Lee, J.-S. & Oh, T.-K.(2006a).Brevundimonas kwangchunensis sp. nov., isolated from an alkaline soil in Korea. Int J Syst Evol Microbiol56, 613–617.[CrossRef][Google Scholar]
Yoon, J.-H., Kang, S.-J., Lee, J.-S. & Oh, T.-K.(2006b).Brevundimonas terrae sp. nov., isolated from an alkaline soil in Korea. Int J Syst Evol Microbiol56, 2915–2919.[CrossRef][Google Scholar]
Yoon, J.-H., Kang, S.-J., Lee, J.-S., Oh, H. W. & Oh, T.-K.(2007).Brevundimonas lenta sp. nov., isolated from soil. Int J Syst Evol Microbiol57, 2236–2240.[CrossRef][Google Scholar]
Brevundimonas naejangsanensis sp. nov., a proteolytic bacterium isolated from soil, and reclassification of Mycoplana bullata into the genus Brevundimonas as Brevundimonas bullata comb. nov.
Supplementary Table S1. Biolog assimilation data
for
Brevundimonas naejangsanensis sp. nov. BIO-TAS2-2
T, species of the genus
Brevundimonas and
Mycoplana bullata. [
PDF] 95 KB