1887

Abstract

A Gram-negative, motile and rod-shaped bacterial strain, BIO-TAS2-2, of the class , was isolated from a soil in Korea and studied using a polyphasic taxonomic approach. Strain BIO-TAS2-2 grew optimally at pH 7.5–8.5 and 30 °C and in the presence of 0–1.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain BIO-TAS2-2 fell within the clade comprising species of the genus , forming a coherent cluster with KSL-145 and LMG 2089. It exhibited 16S rRNA gene sequence similarity values of 96.0–98.7 % to members of the genus and IAM 13153. Strain BIO-TAS2-2 contained Q-10 as the predominant ubiquinone and cyclo-C 7 and C as the major fatty acids. The DNA G+C content was 67.0 mol%. Strain BIO-TAS2-2 exhibited DNA–DNA relatedness levels of 12–19 % with the type strains of phylogenetically related species and . . The novel strain could be differentiated from species and . by differences in phenotypic characteristics. On the basis of phenotypic, phylogenetic and genetic data, strain BIO-TAS2-2 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BIO-TAS2-2 (=KCTC 22631=CCUG 57609). In this study, it is also proposed that be transferred to the genus as comb. nov. (type strain TK0051=ATCC 4278=DSM 7126=JCM 20846=LMG 17157).

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2009-12-01
2020-01-20
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References

  1. Abraham, W.-R., Strömpl, C., Meyer, H., Lindholst, S., Moore, E. R. B., Christ, R., Vancanneyt, M., Tindall, B. J., Bennasar, A. & other authors ( 1999; ). Phylogeny and polyphasic taxonomy of Caulobacter species. Proposal of Maricaulis gen. nov. with Maricaulis maris (Poindexter) comb. nov. as the type species, and emended description of the genera Brevundimonas and Caulobacter. Int J Syst Bacteriol 49, 1053–1073.[CrossRef]
    [Google Scholar]
  2. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the identification of medical bacteria. London: Cambridge University Press.
  3. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  4. Fritz, I., Strömpl, C., Nikitin, D. I., Lysenko, A. M. & Abraham, W.-R. ( 2005; ). Brevundimonas mediterranea sp. nov., a non-stalked species from the Mediterranean Sea. Int J Syst Evol Microbiol 55, 479–486.[CrossRef]
    [Google Scholar]
  5. Gray, P. H. H. & Thornton, H. G. ( 1928; ). Soil bacteria that decompose certain aromatic compounds. Zentralbl Bakteriol Parasitenkd Infektionskr Hyg Abt II 73, 74–96.
    [Google Scholar]
  6. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  7. Lányí, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  8. Li, Y., Kawamura, Y., Fujiwara, N., Naka, T., Liu, H., Huang, X., Kobayashi, K. & Ezaki, T. ( 2004; ). Sphingomonas yabuuchiae sp. nov. and Brevundimonas nasdae sp. nov., isolated from the Russian space laboratory Mir. Int J Syst Evol Microbiol 54, 819–825.[CrossRef]
    [Google Scholar]
  9. Palleroni, N. J. ( 1984; ). Genus Pseudomonas Migula 1894. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 141–199. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  10. Poindexter, J. S. ( 1964; ). Biological properties and classification of the Caulobacter group. Bacteriol Rev 28, 231–295.
    [Google Scholar]
  11. Poindexter, J. S. ( 1989; ). Genus Caulobacter Henrici and Johnson 1935. In Bergey's Manual of Systematic Bacteriology, vol. 3, pp. 1924–1939. Edited by J. T. Staley, M. P. Bryant, N. Pfennig & J. G. Holt. Baltimore: Williams & Wilkins.
  12. Ryu, S. H., Park, M., Lee, J. R., Yun, P.-Y. & Jeon, C. O. ( 2007; ). Brevundimonas aveniformis sp. nov., a stalked species isolated from activated sludge. Int J Syst Evol Microbiol 57, 1561–1565.[CrossRef]
    [Google Scholar]
  13. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  14. Segers, P., Vancanneyt, M., Pot, B., Torck, U., Hoste, B., Dewettinck, D., Falsen, E., Kersters, K. & De Vos, P. ( 1994; ). Classification of Pseudomonas diminuta Leifson and Hugh 1954 and Pseudomonas vesicularis Büsing, Döll, and Freytag 1953 in Brevundimonas gen. nov. as Brevundimonas diminuta comb. nov. and Brevundimonas vesicularis comb. nov., respectively. Int J Syst Bacteriol 44, 499–510.[CrossRef]
    [Google Scholar]
  15. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  16. Urakami, T., Oyanagi, H., Araki, H., Suzuki, K.-I. & Komagata, K. ( 1990; ). Recharacterization and emended description of the genus Mycoplana and description of two new species, Mycoplana ramosa and Mycoplana segnis. Int J Syst Bacteriol 40, 434–442.[CrossRef]
    [Google Scholar]
  17. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  18. Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H. ( 1996; ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46, 502–505.[CrossRef]
    [Google Scholar]
  19. Yoon, J.-H., Lee, S. T. & Park, Y.-H. ( 1998; ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef]
    [Google Scholar]
  20. Yoon, J.-H., Kang, K. H. & Park, Y.-H. ( 2003; ). Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53, 449–454.[CrossRef]
    [Google Scholar]
  21. Yoon, J.-H., Kang, S.-J., Oh, H. W., Lee, J.-S. & Oh, T.-K. ( 2006a; ). Brevundimonas kwangchunensis sp. nov., isolated from an alkaline soil in Korea. Int J Syst Evol Microbiol 56, 613–617.[CrossRef]
    [Google Scholar]
  22. Yoon, J.-H., Kang, S.-J., Lee, J.-S. & Oh, T.-K. ( 2006b; ). Brevundimonas terrae sp. nov., isolated from an alkaline soil in Korea. Int J Syst Evol Microbiol 56, 2915–2919.[CrossRef]
    [Google Scholar]
  23. Yoon, J.-H., Kang, S.-J., Lee, J.-S., Oh, H. W. & Oh, T.-K. ( 2007; ). Brevundimonas lenta sp. nov., isolated from soil. Int J Syst Evol Microbiol 57, 2236–2240.[CrossRef]
    [Google Scholar]
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