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Abstract

A Gram-positive, neutrophilic, non-motile and non-spore-forming actinobacterium, strain FS24, was isolated from a bioreactor treating salt-containing wastewater. This isolate grew in the presence of 0–15 % (w/v) NaCl and at 10-37 °C. The optimum NaCl concentration for growth of FS24 was 5 % (w/v) at 37 °C or 1 % (w/v) at 25 °C. Chemotaxonomic analysis revealed MK-9(H) as the predominant menaquinone and the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, four unknown glycolipids, two unknown phospholipids and an unknown lipid. The major fatty acids were anteiso-C, iso-C, iso-C and anteiso-C. The genomic DNA G+C content was 66.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain FS24 clustered with members of the genus , exhibiting high sequence similarity to the 16S rRNA gene sequences of the type strains of (98.9 %) and (98.8 %), respectively. The DNA–DNA relatedness values of strain FS24 to DSM 15665 and DSM 14442 were 54 and 39 %, respectively. On the basis of phenotypic and genotypic data, strain FS24 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FS24 (=CGMCC 1.7064 =JCM 15136).

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2010-03-01
2019-12-13
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vol. , part 3, pp. 495 - 499

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Electron micrograph of of strain FS24 . Cells were grown for 36 h at 30 °C. Bar 1 µm.

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