1887

Abstract

A novel bacterial strain designated CJ25 was isolated from the estuarine wetland of the Han river in Korea. Identification of this strain was carried out on the basis of polyphasic taxonomy. The isolate was Gram-staining-positive, rod-shaped, non-pigmented and motile. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the isolate was closely related to DSM 5051 with 96.1 % similarity. The predominant fatty acids were anteiso-C (50.25 %), iso-C (18.54 %) and iso-C (10.00 %). The major isoprenoid quinone was MK-7. The G+C content of genomic DNA was 50 mol%. According to physiological data and 16S rRNA gene sequence analysis, the isolate was discriminated from related members of the genus . Therefore, strain CJ25 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CJ25 (=KACC 13125 =JCM 15521).

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2010-03-01
2021-10-21
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References

  1. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequence. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  2. Collins M. D. 1985; Analysis of isoprenoid quinones. Methods Microbiol 18:329–366
    [Google Scholar]
  3. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 2005 phylip (phylogeny inference package), version 3.6. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  6. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  7. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  8. Kuroshima K., Sakane T., Takata R., Yokota A. 1996; Bacillus ehimensis sp. nov. and Bacillus chitinolyticus sp. nov., new chitinolytic members of the genus Bacillus . Int J Syst Bacteriol 46:76–80 [CrossRef]
    [Google Scholar]
  9. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  10. Li D., Kim M., Shim W. J., Yim U. H., Oh J. R., Kwon Y.-J. 2004; Seasonal flux of nonylphenol in Han River, Korea. Chemosphere 56:1–6 [CrossRef]
    [Google Scholar]
  11. Lim J.-M., Jeon C. O., Lee J.-C., Xu L.-H., Jiang C.-L., Kim C.-J. 2006; Paenibacillus gansuensis sp. nov., isolated from desert soil of Gansu Province in China. Int J Syst Evol Microbiol 56:2131–2134 [CrossRef]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  13. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athayle M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  14. Nakamura L. K. 1987; Bacillus alginolyticus sp. nov and Bacillus chondroitinus sp. nov., two alginate-degrading species. Int J Syst Bacteriol 37:284–286 [CrossRef]
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  16. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology . pp 607–654 Edited by Gerhart P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  17. Takeda M., Suzuki I., Koizumi J. 2005; Paenibacillus hodogayensis sp. nov., capable of degrading the polysaccharide produced by Sphaerotilus natans . Int J Syst Evol Microbiol 55:737–741
    [Google Scholar]
  18. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
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