Two recently reported bacterial strains that are able to reductively dehalogenate polychlorinated aliphatic alkanes, including 1,2,3-trichloropropane, 1,2-dichloropropane, 1,1,2,2-tetrachloroethane, 1,1,2-trichloroethane and 1,2-dichloroethane, were further characterized to clarify their taxonomic position. The two strains, designated BL-DC-8 and BL-DC-9, were mesophilic, non-spore-forming, non-motile, Gram-negative staining and strictly anaerobic. Cells were irregular cocci, 0.3–0.6 μm in diameter. The two strains were resistant to ampicillin and vancomycin. Hydrogen was utilized as an electron donor. The genomic DNA G+C content of strains BL-DC-8 and BL-DC-9 was 54.0 and 53.8 mol%, respectively. The major cellular fatty acids were C 9, C 9, C and C. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the strains cluster within the phylum , but are related only distantly to all recognized taxa in the phylum. Morphological, physiological and chemotaxonomic traits as well as phylogenetic analysis support the conclusion that these two strains represent a novel species of a new genus in the phylum , for which the name gen. nov., sp. nov. is proposed. The type strain of is BL-DC-9 (=ATCC BAA-1523 =JCM 15061).


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Morphological features of strains BL-DC-8 (A, B) and BL-DC-9 (C, D) observed by scanning electron microscopy (A, C) and transmission electron microscopy (B, D). Bars, 1 µm (A), 0.2 µm (B, D) and 0.5 µm (C).


Phenotypic characteristics of type strains of type species of genera in the phylum . [PDF](96 KB)

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