1887

Abstract

A novel Gram-negative bacteria, named CZ41_10a, was isolated from coastal surface waters of the north-western Mediterranean Sea. Cells were motile, pleomorphic rods, 1.6 μm long and 0.7 μm wide and formed cream colonies on marine agar medium. The G+C content of the genomic DNA was 65 mol%. Phylogenetic analysis of 16S rRNA gene sequences placed the new isolate in the genus , a member of the family , class . Unlike CL-GR58, its closest relative, strain CZ41_10a was unable to grow anaerobically and did not exhibit nitrate reductase activity. On the basis of DNA–DNA hybridization, fatty acid content and physiological and biochemical characteristics, this isolate represents a novel species for which the name sp. nov. is proposed. The type strain is CZ41_10a (=DSM 19539=CIP 109064=MOLA 84). An emended description of the genus is also given.

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2010-01-01
2020-01-28
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References

  1. Agogué, H., Casamayor, E. O., Bourrain, M., Obernosterer, I., Joux, F., Herndl, G. J. & Lebaron, P. ( 2005; ). A survey on bacteria inhabiting the sea surface microlayer of coastal ecosystems. FEMS Microbiol Ecol 54, 269–280.[CrossRef]
    [Google Scholar]
  2. Capone, D. G. & Knapp, N. ( 2007; ). A marine nitrogen cycle fix? Nature 445, 159–160.[CrossRef]
    [Google Scholar]
  3. Goregues, C. M., Michotey, V. D. & Bonin, P. C. ( 2005; ). Molecular, biochemical, and physiological approaches for understanding the ecology of denitrification. Microb Ecol 49, 198–208.[CrossRef]
    [Google Scholar]
  4. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  5. Michotey, V., Mejean, V. & Bonin, P. ( 2000; ). Comparison of methods for quantification of cytochrome cd(1)-denitrifying bacteria in environmental marine samples. Appl Environ Microbiol 66, 1564–1571.[CrossRef]
    [Google Scholar]
  6. Powers, E. M. ( 1995; ). Efficacy of the Ryu nonstaining KOH technique for rapidly determining Gram reactions of food-borne and waterborne bacteria and yeasts. Appl Environ Microbiol 61, 3756–3758.
    [Google Scholar]
  7. Raguénès, G., Christen, R., Guézennec, J., Pignet, P. & Barbier, G. ( 1997; ). Vibrio diabolicus sp. nov., a new polysaccharidic-secreting organism isolated from a deep-sea hydrothermal vent polychaete annelid, Alvinella pompejana. Int J Syst Bacteriol 47, 989–995.[CrossRef]
    [Google Scholar]
  8. Rosch, C., Mergel, A. & Bothe, H. ( 2002; ). Biodiversity of denitrifying and dinitrogen-fixing bacteria in an acid forest soil. Appl Environ Microbiol 68, 3818–3829.[CrossRef]
    [Google Scholar]
  9. Rotthauwe, J. H., Witzel, K. P. & Liesack, W. ( 1997; ). The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations. Appl Environ Microbiol 63, 4704–4712.
    [Google Scholar]
  10. Tang, Y. W., Ellis, N. M., Hopkins, M. K., Smith, D. H., Dodge, D. E & Persing, D. H ( 1998; ). Comparison of phenotypic and genotypic techniques for identification of unusual aerobic pathogenic gram-negative bacilli. J Clin Microbiol 36, 3674–3679.
    [Google Scholar]
  11. Urios, L., Agogué, H., Lesongeur, F., Stackebrandt, E. & Lebaron, P. ( 2006; ). Balneola vulgaris gen. nov., sp. nov., a member of the phylum Bacteroidetes from the north-western Mediterranean Sea. Int J Syst Evol Microbiol 56, 1883–1887.[CrossRef]
    [Google Scholar]
  12. Urios, L., Michotey, V., Intertaglia, L., Lesongeur, F. & Lebaron, P. ( 2008; ). Nisaea denitrificans gen. nov., sp. nov. and Nisaea nitritireducens sp. nov., two new members of the Alphaproteobacteria from the Mediterranean Sea. Int J Syst Evol Microbiol 58, 2336–2341.[CrossRef]
    [Google Scholar]
  13. Wery, N., Lesongeur, F., Pignet, P., Derennes, V., Cambon-Bonavita, M., Godfroy, A. & Barbier, G. ( 2001a; ). Marinitoga camini gen. nov., sp. nov., a rod-shaped bacterium belonging to the order Thermotogales, isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 51, 495–504.
    [Google Scholar]
  14. Wery, N., Moricet, J., Cueff, V., Jean, J., Pignet, P., Lesongeur, F., Cambon-Bonavita, M. & Barbier, G. ( 2001b; ). Caloranaerobacter azorensis gen. nov., sp. nov., an anaerobic thermophilic bacterium isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 51, 1789–1796.[CrossRef]
    [Google Scholar]
  15. Zehr, J. P. & McReynolds, L. A. ( 1989; ). Use of degenerate oligonucleotides for amplification of the nifH gene from the marine cyanobacterium Trichodesmium thiebautii. Appl Environ Microbiol 55, 2522–2526.
    [Google Scholar]
  16. Zhang, G. I., Hwang, C. Y. & Cho, B. C. ( 2008; ). Thalassobaculum litoreum gen. nov., sp. nov., a member of the family Rhodospirillaceae isolated from coastal seawater. Int J Syst Evol Microbiol 58, 479–485.[CrossRef]
    [Google Scholar]
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vol. , part 1, pp. 209 - 213

Effects of temperature, pH and NaCl concentration on the growth of strain CZ41_10a . [ PDF] 35 KB



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