1887

Abstract

Two strains of Gram-positive cocci were isolated from viscera of common voles ( Pallas) with generalized infection in the Czech Republic. Biochemical features and phylogenetic analysis based on 16S rRNA gene sequences showed that the strains are representatives of the genus and assigned as the nearest relative. A detailed characterization done by ribotyping, and gene sequencing, whole-cell protein analysis and rep-PCR using the (GTG) primer differentiated the two strains from all described staphylococci. DNA–DNA hybridization with the type strain of demonstrated that the two strains should be considered as members of a novel species (26.8 % reassociation). The two analysed strains were found to be coagulase-negative, novobiocin-susceptible, oxidase-negative cultures, phenotypically close to one another, but showing differences in ribotype profiles. The major fatty acids were iso-C, iso-C, anteiso-C, C 6,9/anteiso-C, C and C 9. MK-7 was the predominant isoprenoid quinone, with minor amounts of MK-6 and MK-8. The polar lipid profile was composed of the major lipids diphosphatidylglycerol and phosphatidylglycerol and several unknown lipids. These results proved that the two isolates represent a novel staphylococcal species. The name proposed for this novel taxon is sp. nov.; the type strain is 4005-LJ(m) (=CCM 4903 =CCUG 55861 =DSM 22147).

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2010-03-01
2019-12-12
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(GTG) PCR pattern cluster analysis of strains CCM 4903 and CCM 4904 and the most closely related staphylococcal species.

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Whole-cell protein cluster analysis of strains CCM 4903 and CCM 4904 and the most closely related staphylococcal species.

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Polar lipid profile of strain CCM 4904 after two-dimensional TLC and detection with molybdatophosphoric acid. DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; APL1, 2, unknown aminophospholipids; PL1, unknown phospholipid; GL1, 2, unknown glycolipids; L1, unknown polar lipid.

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