The novel actinomycete strain Ap1 was isolated from rhizosphere soil of the argan tree ( L.) in the south of Morocco. Strain Ap1 has been reported as a novel producer of the pentaene polyene macrolide isochainin, which strongly inhibits the growth of pathogenic yeasts and phytopathogenic fungi. Strain Ap1 shows a greyish-white aerial mycelium with chains of smooth-surfaced spores of the type and a cell wall containing -diaminopimelic acid. Based on chemotaxonomy and morphological features, strain Ap1 was identified as a member of the genus . 16S rRNA gene sequence similarities based on almost-complete 16S rRNA gene sequences showed that strain Ap1 is closely associated with members of the species group (, , , ‘’, ‘’, and ) and others (, , , and ). However, protein profiling, DNA–DNA hybridization and BOX-PCR fingerprinting proved a relationship above the species level. In addition, the phenotype also allowed for the differentiation of strain Ap1 from its closest neighbours. As a result of this polyphasic approach, we conclude that strain Ap1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Ap1 (=R-22003 =LMG 23016 =DSM 41918).


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vol. , part 11, pp. 2857 - 2863

Physiological characteristics of strain Ap1 and its phylogenetic neighbours. [PDF](89 KB)

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