sp. nov., isolated from seaweed, and emended description of the genus Free

Abstract

An aerobic, Gram-reaction-negative, non-motile, catalase- and oxidase-positive bacterium, designated strain MDSW-25, was isolated from seaweed collected in the vicinity of Mara Island in Jeju province, Republic of Korea. Colonies were smooth, circular and convex with entire edges and yellow in colour. Growth occurred at 10–30 °C, at pH 6.1–9.1 and in the presence of 1–12 % (w/v) NaCl. The major fatty acids were iso-C (25.6 %) and iso-C G (11.3 %), and the major menaquinone was MK-6. The DNA G+C content was 30.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain MDSW-25 belonged to the genus , family . Sequence similarity with and was 97.5 and 95.4 %, respectively, but DNA relatedness between strain MDSW-25 and KCTC 12708 was only 47 %. A battery of phenotypic data, phylogenetic inference and DNA–DNA hybridization analyses supports the conclusion that strain MDSW-25 (=KCTC 22373 =DSM 21425) represents a novel species of the genus , for which the name sp. nov. is proposed. On the basis of new data obtained in this study, an emended description of the genus is also proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.011338-0
2010-03-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/3/591.html?itemId=/content/journal/ijsem/10.1099/ijs.0.011338-0&mimeType=html&fmt=ahah

References

  1. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
    [Google Scholar]
  2. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376
    [Google Scholar]
  3. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  4. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  5. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  6. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20) pp 173–199 Edited by Goodfellow M., Minnikin D. E. New York: Academic Press;
    [Google Scholar]
  7. Lee S. D. 2006; Phycicoccus jejuensis gen. nov. sp. nov. an actinomycete isolated from seaweed. Int J Syst Evol Microbiol 56:2369–2373 [CrossRef]
    [Google Scholar]
  8. Lee S. D. 2007; Tamlana crocina gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae , isolated from beach sediment in Korea. Int J Syst Evol Microbiol 57:764–769 [CrossRef]
    [Google Scholar]
  9. Lee S. D., Kang S.-O., Hah Y. C. 2000; Hongia gen. nov., a new genus of the order Actinomycetales . Int J Syst Evol Microbiol 50:191–199 [CrossRef]
    [Google Scholar]
  10. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  11. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117 [CrossRef]
    [Google Scholar]
  12. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  13. Nedashkovskaya O. I., Kim S. B., Han S. K., Lysenko A. M., Rohde M., Zhukova N. V., Falsen E., Frolova G. M., Mikhailov V. V., Bae K. S. 2003; Mesonia algae gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the green alga Acrosiphonia sonderi (Kütz) Kornm. Int J Syst Evol Microbiol 53:1967–1971 [CrossRef]
    [Google Scholar]
  14. Nedashkovskaya O. I., Kim S. B., Zhukova N. V., Kwak J., Mikhailov V. V., Bae K. S. 2006; Mesonia mobilis sp. nov., isolated from seawater, and emended description of the genus Mesonia . Int J Syst Evol Microbiol 56:2433–2436 [CrossRef]
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  16. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  17. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  18. Weon H.-Y., Kim B.-Y., Son J.-A., Jang H. B., Hong S. K., Go S.-J., Kwon S.-W. 2008; Massilia aerilata sp. nov., isolated from an air sample. Int J Syst Evol Microbiol 58:1422–1425 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.011338-0
Loading
/content/journal/ijsem/10.1099/ijs.0.011338-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed