1887

Abstract

A Gram-negative, strictly aerobic bacterium, comprising non-endospore-forming motile rods (1.2–2.0 μm × 0.4–0.6 μm) with polar flagellae was isolated from root nodules of the leguminous plant (Willd.) Ohwi growing on the coast of Yakushima Island, Kagoshima Prefecture, Japan. The novel strain, designated Yak96B, grew at an optimum pH of 7.0 and an optimum temperature of 28 °C. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the new strain was closely related to J1 and IPL18, with sequence similarities of 98.1 % and 97.8 %, respectively. However, the DNA–DNA relatedness values of strain Yak96B with LMG 21357 and CCM 7426 were 53.6 % and 34 %, respectively. The DNA G+C content of strain Yak96B was 65.3 mol%, the predominant isoprenoid quinone was Q10 (85 %) and the polar lipids were phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids (>5 %) were 11-methyl C 7 (35.0 %), C (22.4 %), C 7 (21.8 %), C cyclo 8 (6.8 %) and C (5.4 %). The infection/nodulation test was negative and and genes were not detected. Based on its chemotaxonomic and physiological characteristics, strain Yak96B represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Yak96B (=KCTC 22147=NBRC 103855=LMG 24299).

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2010-03-01
2021-04-10
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