1887

Abstract

A Gram-negative, non-motile, non-spore-forming coccoid bacterium (strain BO1) was isolated recently from a breast implant infection of a 71-year-old female patient with clinical signs of brucellosis. Affiliation of strain BO1 to the genus was confirmed by means of polyamine pattern, polar lipid profile, fatty acid profile, quinone system, DNA–DNA hybridization studies and by insertion sequence (IS)-specific PCR. Strain BO1 harboured four to five copies of the -specific insertion element IS, displaying a unique banding pattern, and exhibited a unique 16S rRNA gene sequence and also grouped separately in multilocus sequence typing analysis. Strain BO1 reacted with M-monospecific antiserum. Incomplete lysis was detected with bacteriophages Tb (Tbilisi), F1 and F25. Biochemical profiling revealed a high degree of enzymic activity and metabolic capabilities. In multilocus VNTR (variable-number tandem-repeat) analysis, strain BO1 showed a very distinctive profile and clustered with the other ‘exotic’ strains, including strains isolated from marine mammals, and , biovar 5 and . Comparative and gene sequence analysis revealed the most divergent sequences identified to date for a strain. The gene sequence of strain BO1 differed in seven nucleotides from the consensus sequence. Using the species-specific multiplex PCR assay, strain BO1 displayed a unique banding pattern not observed in other species. From the phenotypic and molecular analysis it became evident that strain BO1 was clearly different from all other species, and therefore represents a novel species within the genus . Because of its unexpected isolation, the name with the type strain BO1 (=BCCN 09-01=CPAM 6436) is proposed.

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2010-04-01
2020-12-02
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References

  1. Al Dahouk S., Fleche P. L., Noeckler K., Jacques I., Grayon M., Scholz H. C., Tomaso H., Vergnaud G., Neubauer H. 2007; Evaluation of Brucella MLVA typing for human brucellosis. J Microbiol Methods 69:137–145 [CrossRef]
    [Google Scholar]
  2. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J. 1996; Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47:39–52 [CrossRef]
    [Google Scholar]
  3. Bricker B. J., Halling S. M. 1994; Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis and Brucella suis bv. 1 by PCR. J Clin Microbiol 32:2660–2666
    [Google Scholar]
  4. Bricker B. J., Ewalt D. R., Halling S. M. 2003; Brucella ‘HOOF-Prints': strain typing by multi-locus analysis of variable number tandem repeats (VNTRs. BMC Microbiol 11:3–15
    [Google Scholar]
  5. Buddle M. B. 1956; Studies on Brucella ovis (n. sp.), a cause of genital disease of sheep in New Zealand and Australia. J Hyg (Lond 54:351–364 [CrossRef]
    [Google Scholar]
  6. Carmichael L. E., Bruner D. W. 1968; Characteristics of a newly-recognized species of Brucella responsible for infectious canine abortions. Cornell Vet 48:579–592
    [Google Scholar]
  7. Crasta O. R., Folkerts O., Fei Z., Mane S. P., Evans C., Martino-Catt S., Bricker B., Yu G., Du L., Sobral B. W. 2008; Genome sequence of Brucella abortus vaccine strain S19 compared to virulent strains yields candidate virulence genes. PLoS One 3:e2193 [CrossRef]
    [Google Scholar]
  8. De B. K., Stauffer L., Koylass M. S., Sharp S. E., Gee J. E., Helsel L. O., Steigerwalt A. G., Vega R., Clark T. A. other authors 2008; Novel Brucella strain (BO1) associated with a prosthetic breast implant infection. J Clin Microbiol 46:43–49 [CrossRef]
    [Google Scholar]
  9. DelVecchio V. G., Kapatral V., Redkar R. J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A. other authors 2002; The genome sequence of the facultative intracellular pathogen Brucella melitensis . Proc Natl Acad Sci U S A 99:443–448 [CrossRef]
    [Google Scholar]
  10. Foster G., Osterman B., Godfroid J., Jacques I., Cloeckaert A. 2007; Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. Int J Syst Evol Microbiol 57:2688–2693 [CrossRef]
    [Google Scholar]
  11. Garcia-Yoldi D., Marin C. M., de Miguel M. J., Munoz P. M., Vizmanos J. L., Lopez-Goni I. 2006; Multiplex PCR assay for the identification and differentiation of all Brucella species and the vaccine strains Brucella abortus S19 and RB51 and Brucella melitensis Rev1. Clin Chem 52:779–781 [CrossRef]
    [Google Scholar]
  12. Gee J. E., De B. K., Levett P. N., Whitney A. M., Novak R. T., Popovic T. 2004; Use of 16S rRNA gene sequencing for rapid confirmatory identification of Brucella isolates. J Clin Microbiol 42:3649–3654 [CrossRef]
    [Google Scholar]
  13. Halling S. M., Peterson-Burch B. D., Bricker B. J., Zuerner R. L., Qing Z., Li L. L., Kapur V., Alt D. P., Olsen S. C. 2005; Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis . J Bacteriol 187:2715–2726 [CrossRef]
    [Google Scholar]
  14. Hubalek Z., Scholz H. C., Sedlaceck I., Melzer F., Sanogo Y., Nesvadbova J. 2007; Brucellosis of the Common Vole ( Microtus arvalis . Vector Borne Zoonotic Dis 7:679–687
    [Google Scholar]
  15. Huddleson I. F. 1929; The differentiation of the species of the genus Brucella . Michigan State College Agricultural Experimental Station Technical Bulletin 100:1–16
    [Google Scholar]
  16. Jevitt L. A., Weigel L. M., De B., Popovic T., Patel J. B. 2005; Abstr. C-357.. Abstr. 105th Gen. Meet. Am. Soc. Microbiol Washington, DC: American Society for Microbiology;
    [Google Scholar]
  17. Le Flèche P., Jacques I., Grayon M., Al Dahouk S., Bouchon P., Denoeud F., Nockler K., Neubauer H., Guilloteau L. A. other authors 2006; Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay. BMC Microbiol 9:6–9
    [Google Scholar]
  18. Meyer K. F., Shaw E. B. 1920; A comparison of the morphologic, cultural and biochemical characteristics of B. abortus and B. melitensis from cattle. Studies on the genus Brucella nov. gen. J Infect Dis 27:173–184 [CrossRef]
    [Google Scholar]
  19. Osterman B., Moriyón I. 2006; International Committee on Systematics of Prokaryotes; Subcommittee on the Taxonomy of Brucella: minutes of the meeting, 17 September 2003, Pamplona, Spain. Int J Syst Evol Microbiol 56:1173–1175 [CrossRef]
    [Google Scholar]
  20. Paulsen I. T., Seshadri R., Nelson K. E., Eisen J. A., Heidelberg J. F., Read T. D., Dodson R. J., Umayam L., Brinkac L. M. other authors 2002; The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts. Proc Natl Acad Sci U S A 99:13148–13153 [CrossRef]
    [Google Scholar]
  21. Scholz H. C., Hubalek Z., Sedlácek I., Vergnaud G., Tomaso H., Al Dahouk S., Melzer F., Kämpfer P., Neubauer H. other authors 2008a; Brucella microti sp. nov., isolated from the common vole Microtus arvalis . Int J Syst Evol Microbiol 58:375–382 [CrossRef]
    [Google Scholar]
  22. Scholz H. C., Al Dahouk S., Tomaso H., Neubauer H., Witte A., Schloter M., Kämpfer P., Falsen E., Pfeffer M., Engel M. 2008b; Genetic diversity and phylogenetic relationships of bacteria belonging to the Ochrobactrum-Brucella group by recA and 16S rRNA gene-based comparative sequence analysis. Syst Appl Microbiol 31:1–16 [CrossRef]
    [Google Scholar]
  23. Scholz H. C., Hubalek Z., Nesvadbova J., Tomaso H., Vergnaud G., Le Flèche P., Whatmore A. M., Al Dahouk S., Krüger M. other authors 2008c; Isolation of Brucella microti from soil. Emerg Infect Dis 14:1316–1317 [CrossRef]
    [Google Scholar]
  24. Scholz H. C., Hofer E., Vergnaud G., Le Flèche P., Whatmore A. M., Al Dahouk S., Pfeffer M., Krüger M., Cloeckaert A., Tomaso H. 2009; Isolation of Brucella microti from mandibular lymph nodes of red foxes, Vulpes vulpes , in Lower Austria. Vector Borne Zoonotic Dis 9:153–156 [CrossRef]
    [Google Scholar]
  25. Stoenner H. G., Lackman D. B. 1957; A new species of Brucella isolated from the desert wood rat, Neotoma lepida Thomas. Am J Vet Res 18:947–951
    [Google Scholar]
  26. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  27. Tindall B. J. 1990a; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [CrossRef]
    [Google Scholar]
  28. Tindall B. J. 1990b; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [CrossRef]
    [Google Scholar]
  29. Verger J.-M., Grimont F., Grimont P. A. D., Grayon M. 1985; Brucella , a monospecific genus as shown by deoxyribonucleic acid hybridization. Int J Syst Bacteriol 35:292–295 [CrossRef]
    [Google Scholar]
  30. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  31. Whatmore A. M., Perrett L. L., Macmillan A. P. 2007; Characterisation of the genetic diversity of Brucella by multilocus sequencing. BMC Microbiol 7:34 [CrossRef]
    [Google Scholar]
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