1887

Abstract

Strains YIM 90625, YIM 90630, YIM 90636 and YIM 90694 were isolated from a salt lake in Xinjiang province, north-west China, and were subjected to a polyphasic analysis to determine their taxonomic positions. All isolates were moderately halophilic and were able to grow at NaCl concentrations up to 15 or 20 % (w/v). The genomic DNA G+C contents of the strains ranged from 69.1 to 70.1 mol%. Based on the results of 16S rRNA gene sequence analysis, DNA–DNA relatedness, phenotypic characteristics and chemotaxonomic data, the isolates are proposed to represent four novel species of the genus for which the names sp. nov. (type strain YIM 90625=CCTCC AA 208051=DSM 45264), sp. nov. (type strain YIM 90630=CCTCC AA 208052=DSM 45265), sp. nov. (type strain YIM 90636=CCTCC AA 208053=DSM 45266) and sp. nov. (type strain YIM 90694=CCTCC AA 208054=DSM 45267).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.011122-0
2009-12-01
2024-12-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/12/2923.html?itemId=/content/journal/ijsem/10.1099/ijs.0.011122-0&mimeType=html&fmt=ahah

References

  1. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W.(2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef] [Google Scholar]
  2. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  3. Di Marco, A. & Spalla, C.(1957). La produzione di cobalamine da fermentazione con una nuova specie di Nocardia: Nocardia rugosa. Giorn Microbiol 4, 24–30 (in Italian). [Google Scholar]
  4. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  5. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  6. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  7. Hasegawa, T., Takizawa, M. & Tanida, S.(1983). A rapid analysis for chemical grouping aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef] [Google Scholar]
  8. Huß, V. A. R., Festl, H. & Schleifer, K. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef] [Google Scholar]
  9. Jahnke, K. D.(1992).basic computer program for evaluation of spectroscopic DNA renaturation data from GILFORD SYSTEM 2600 spectrophotometer on a PC/XT/AT type personal computer. J Microbiol Methods 15, 61–73.[CrossRef] [Google Scholar]
  10. Kelly, K. L.(1964).Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office.
  11. Kim, S. B. & Goodfellow, M.(1999). Reclassification of Amycolatopsisrugosa Lechevalier et al. 1986 as Prauserella rugosa gen. nov., comb. nov. Int J Syst Bacteriol 49, 507–512.[CrossRef] [Google Scholar]
  12. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequence. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  13. Kroppenstedt, R. M.(1982). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5, 2359–2367.[CrossRef] [Google Scholar]
  14. Lechevalier, M. P., Prauser, H., Labeda, D. P. & Ruan, J.-S.(1986). Two new genera of nocardioform actinomycetes: Amycolata gen. nov. and Amycolatopsis gen. nov. Int J Syst Bacteriol 36, 29–37.[CrossRef] [Google Scholar]
  15. Li, W.-J., Xu, P., Tang, S.-K., Xu, L.-H., Kroppenstedt, R.-M., Stackebrandt, E. & Jiang, C.-L.(2003).Prauserella halophila sp. nov. and Prauserella alba sp. nov., moderately halophilic actinomycetes from saline soil. Int J Syst Evol Microbiol 53, 1545–1549.[CrossRef] [Google Scholar]
  16. Li, W. J., Xu, P., Schumann, P., Zhang, Y. Q., Pukall, R., Xu, L. H., Stackebrandt, E. & Jiang, C. L.(2007).Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57, 1424–1428.[CrossRef] [Google Scholar]
  17. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  18. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  19. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  20. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  21. Sasser, M.(1990). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16 [Google Scholar]
  22. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  23. Tamura, K., Dudley, J., Nei, M. & Kumar, S.(2007).mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef] [Google Scholar]
  24. Tang, S.-K., Tian, X.-P., Zhi, X.-Y., Cai, M., Wu, J.-Y., Yang, L.-L., Xu, L.-H. & Li, W.-J.(2008).Haloactinospora alba gen. nov., sp. nov., a halophilic filamentous actinomycete of the family Nocardiopsaceae. Int J Syst Evol Microbiol 58, 2075–2080.[CrossRef] [Google Scholar]
  25. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  26. Williams, S. T., Goodfellow, M., Alderson, G., Wellington, E. M. H., Sneath, P. H. A. & Sackin, M. J.(1983). Numerical classification of Streptomyces and related genera. J Gen Microbiol 129, 1743–1813. [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.011122-0
Loading
/content/journal/ijsem/10.1099/ijs.0.011122-0
Loading

Data & Media loading...

Supplements

Scanning electron micrographs of cells of the four novel isolates. [PDF](499 KB)

PDF

Culture characteristics of the four novel isolates. [PDF](15 KB)

PDF

Menaquinone patterns of the four novel isolates. [PDF](16 KB)

PDF

Fatty acid profiles of the four novel isolates. [PDF](19 KB)

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error