An obligately halophilic, stalked bacterium, designated strain GSW-23, was isolated from seawater that had been collected on the coast of Jeju, Korea. Cells of the strain were characteristically Gram-negative, aerobic, chemo-organotrophic, non-budding, motile rods or vibrioids that possessed prosthecae and holdfasts. Multiplication occurred by means of binary fission. The major ubiquinone was Q-10. The dominant cellular fatty acids were summed feature 7 (one or more of C 9, C 12 and C 7; 37.1 %), C (25.5 %) and C (14.1 %). The DNA G+C content was 53.3 mol%. 16S rRNA gene sequence analyses showed that the organism was related to members of the family and formed a distinct clade between members of the genus and DSM 5838. Strain GSW-23 was most closely related to the genus (92.5–93.9 % sequence similarity), but differed from members of the genus by reproduction by binary fission, some physiological properties (gelatin liquefaction and tolerance of 6 % NaCl) and chemotaxonomic features (major fatty acids, major quinones and DNA G+C content). The other genera of the family can be readily differentiated from the isolate by a battery of cultural, physiological and chemotaxonomic characteristics. On the basis of the phenotypic and phylogenetic data presented here, strain GSW-23 represents a novel genus and species in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is strain GSW-23 (=KCTC 22146 =DSM 19734 =JCM 14975).


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  1. Abraham, W. R., Strömpl, C., Meyer, H., Lindholst, S., Moore, E. R., Christ, R., Vancanneyt, M., Tindall, B. J., Bennasar, A. & other authors(1999). Phylogeny and polyphasic taxonomy of Caulobacter species. Proposal of Maricaulis gen. nov. with Maricaulis maris (Poindexter) comb. nov. as the type species, and emended description of the genera Brevundimonas and Caulobacter. Int J Syst Bacteriol 49, 1053–1073.[CrossRef] [Google Scholar]
  2. Abraham, W. R., Strömpl, C., Bennasar, A., Vancanneyt, M., Snauwaert, C., Swings, J., Smit, J. & Moore, E. R. B.(2002). Phylogeny of Maricaulis Abraham et al. 1999 and proposal of Maricaulis virginensis sp. nov., M. parjimensis sp. nov., M. washingtonensis sp. nov. and M. salignorans sp. nov. Int J Syst Evol Microbiol 52, 2191–2201.[CrossRef] [Google Scholar]
  3. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  4. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  5. Kroppenstedt, R. M.(1985). Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20), pp. 173–199. Edited by M. Goodfellow & D. E. Minnikin. New York: Academic Press.
  6. Lee, S. D.(2007).Tamlana crocina gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from beach sediment in Korea. Int J Syst Evol Microbiol 57, 764–769.[CrossRef] [Google Scholar]
  7. Lee, K. B., Liu, C. T., Anzai, Y., Kim, H., Aono, T. & Oyaizu, H.(2005). The hierarchical system of the ‘Alphaproteobacteria’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov. Int J Syst Evol Microbiol 55, 1907–1919.[CrossRef] [Google Scholar]
  8. Lee, K., Lee, H. K., Choi, T.-H. & Cho, J.-C.(2007).Robiginitomaculum antarcticum gen. nov., sp. nov., a member of the family Hyphomonadaceae, from Antarctic seawater. Int J Syst Evol Microbiol 57, 2595–2599.[CrossRef] [Google Scholar]
  9. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  10. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  11. Moore, R. L., Weiner, R. M. & Gebers, R.(1984). Genus Hyphomonas Pongratz 1957 nom. rev. emend., Hyphomonas polymorpha Pongratz 1957 nom. rev. emend., and Hyphomonas neptunium (Liefson 1964) comb. nov. emend. (Hyphomicrobium neptunium). Int J Syst Bacteriol 34, 71–73.[CrossRef] [Google Scholar]
  12. Pongratz, E.(1957). D'une bactérie pédiculée isolée d'un pus de sinus. Schweiz Z Pathol Bakteriol 20, 593–608 (in French). [Google Scholar]
  13. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  14. Schlesner, H., Bartels, C., Sittig, M., Dorsch, M. & Stackebrandt, E.(1990). Taxonomic and phylogenetic studies on a new taxon of budding, hyphal Proteobacteria, Hirschia baltica gen. nov., sp. nov. Int J Syst Bacteriol 40, 443–451.[CrossRef] [Google Scholar]
  15. Strömpl, C., Hold, G. N., Lunsdorf, H., Graham, J., Gallacher, S., Abraham, W.-R., Moore, E. R. B. & Timmis, K. N.(2003).Oceanicaulis alexandrii gen. nov., sp. nov., a novel stalked bacterium isolated from a culture of the dinoflagellate Alexandrium tamarense (Lebour) Balech. Int J Syst Evol Microbiol 53, 1901–1906.[CrossRef] [Google Scholar]
  16. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  17. Weiner, R. M., Devine, R. A., Powell, D. M., Dagasan, L. & Moore, R. L.(1985).Hyphomonas oceanitis sp. nov., Hyphomonas hirschiana sp. nov., and Hyphomonas jannaschiana sp. nov. Int J Syst Bacteriol 35, 237–243.[CrossRef] [Google Scholar]
  18. Weiner, R. M., Melick, M., O'Neill, K. & Quintero, E.(2000).Hyphomonas adhaerens sp. nov., Hyphomonas johnsonii sp. nov. and Hyphomonas rosenbergii sp. nov., marine budding and prosthecate bacteria. Int J Syst Evol Microbiol 50, 459–469.[CrossRef] [Google Scholar]

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vol. , part 12, pp. 2951 - 2955

Cellular fatty acid compositions of strain GSW-23 and the type strains of the type species of related genera of the family . [ PDF] 74 KB

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