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Abstract

An obligately halophilic, stalked bacterium, designated strain GSW-23, was isolated from seawater that had been collected on the coast of Jeju, Korea. Cells of the strain were characteristically Gram-negative, aerobic, chemo-organotrophic, non-budding, motile rods or vibrioids that possessed prosthecae and holdfasts. Multiplication occurred by means of binary fission. The major ubiquinone was Q-10. The dominant cellular fatty acids were summed feature 7 (one or more of C 9, C 12 and C 7; 37.1 %), C (25.5 %) and C (14.1 %). The DNA G+C content was 53.3 mol%. 16S rRNA gene sequence analyses showed that the organism was related to members of the family and formed a distinct clade between members of the genus and DSM 5838. Strain GSW-23 was most closely related to the genus (92.5–93.9 % sequence similarity), but differed from members of the genus by reproduction by binary fission, some physiological properties (gelatin liquefaction and tolerance of 6 % NaCl) and chemotaxonomic features (major fatty acids, major quinones and DNA G+C content). The other genera of the family can be readily differentiated from the isolate by a battery of cultural, physiological and chemotaxonomic characteristics. On the basis of the phenotypic and phylogenetic data presented here, strain GSW-23 represents a novel genus and species in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is strain GSW-23 (=KCTC 22146 =DSM 19734 =JCM 14975).

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2009-12-01
2019-10-22
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vol. , part 12, pp. 2951 - 2955

Cellular fatty acid compositions of strain GSW-23 and the type strains of the type species of related genera of the family . [ PDF] 74 KB



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